FB2024_03 , released June 25, 2024
Allele: Dmel\nbsEY15506
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General Information
Symbol
Dmel\nbsEY15506
Species
D. melanogaster
Name
FlyBase ID
FBal0178690
Feature type
allele
Associated gene
Dmel\nbs
Associated Insertion(s)
P{EPgy2}nbsEY15506
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Mukherjee et al., 2009)
Mutagen
P-element activity
(Gene Disruption Project members, 2001-)
Progenitor genotype
Associated Insertion(s)
P{EPgy2}nbsEY15506
Cytology
Description

Insertion in the second exon, within coding sequences. Transcription in this mutant initiates within the P-element, just downstream of the 3' end of the w sequences. Translation is predicted to begin at the last three nucleotides of P-element sequence and continue in-frame into nbs sequences. In the predicted translation product, the first residue of the first BRCT domain is changed from Leu to Met, but the domain is otherwise intact. The nbs N-terminal FHA domain is missing in this product as the insertion occurs downstream of the sequence coding this domain.

(Mukherjee et al., 2009)
Allele components
Component
Use(s)
Inserted element
P{EPgy2}
Regulatory region(s)
UASp
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      nbs1/nbsEY15506 larvae are hypersensitive to gamma irradiation compared to controls. Homozygous larvae are also sensitive to gamma irradiation compared to controls, but are significantly less sensitive than nbs1/nbsEY15506 larvae at doses higher than 500 rads.

      Homozygotes have a normal G2/M DNA damage-dependent cell cycle checkpoint induced by irradiation at high-dose (4000 rads). At low-dose irradiation (1000 rads) the checkpoint is significantly reduced compared to wild type.

      nbs1/nbsEY15506 larvae show loss of the G2/M DNA damage-dependent cell cycle checkpoint at low-dose irradiation (1000 rads) and a significant weakening of the checkpoint at high-dose irradiation (4000 rads).

      (Mukherjee et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      nbsEY15506
      nbsP
      (Mukherjee et al., 2009)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)