FlyBase Allele Report: Dmel\seq[UAS.cBa]

General Information

Symbol

Dmel\seq^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Brenman

FlyBase ID

FBal0178045

Feature type

allele

Associated gene

Dmel\seq

Associated Insertion(s)

Carried in Construct

P{UAS-seq.B}

Also Known As

UAS-seq

Key Links

Transgenic product class

wild_type

(Brenman, 2005.8)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brenman, 2005.8)

Mutagen

in vitro construct

(Brenman, 2005.8)

Progenitor genotype

Carried in construct

P{UAS-seq.B}

Cytology

Description

Expression of wild type seq gene is governed by UAS regulatory sequences.

(Brenman, 2005.8)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

seq

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.long

(Petrovic and Hummel, 2008)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^GMR.long

(Petrovic and Hummel, 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Over-expression of seq^Scer\UAS.cBa in a wild-type CNS under the control of Scer\GAL4^elav-C155; Scer\GAL4^sca-109-68 results in the abrogation of ganglionic branching.

(Araújo and Casanova, 2011)

The growth cones seen in the axons of R8/R7 photoreceptor cells of pupal stage flies expressing seq^Scer\UAS.cBa under the control of Scer\GAL4^GMR.long fail to undergo the morphological change from an expanded to a condensed appearance. R8 axons extend towards and terminate precisely in the R7 layer (M6), resulting in a single R8/R7 layer in M6 in the medulla of adult flies.

(Petrovic and Hummel, 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.long, seq^UAS.cBa has abnormal neuroanatomy phenotype, suppressible by CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

NOT Suppressor of

Statement

Reference

seq^UAS.cBa/Scer\GAL4^GMR.long is a non-suppressor of abnormal neuroanatomy phenotype of CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

Other

Statement

Reference

Scer\GAL4^GMR.long, seq^UAS.cBa, sev³ has abnormal neuroanatomy phenotype

(Petrovic and Hummel, 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.long, seq^UAS.cBa has photoreceptor cell R8 phenotype, suppressible by CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

Scer\GAL4^GMR.long, seq^UAS.cBa has medulla layer M6 phenotype, suppressible by CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

Scer\GAL4^GMR.long, seq^UAS.cBa has medulla layer M3 phenotype, suppressible by CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/seq^UAS.cBa is a non-suppressor of embryonic/larval ganglionic tracheal branch phenotype of Scer\GAL4^insc-Mz1407, bnl^UAS.cSa

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^GMR.long is a non-suppressor of photoreceptor cell R7 phenotype of CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

seq^UAS.cBa/Scer\GAL4^GMR.long is a non-suppressor of medulla layer M6 phenotype of CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

seq^UAS.cBa/Scer\GAL4^GMR.long is a non-suppressor of medulla layer M3 phenotype of CadN⁴⁰⁵

(Petrovic and Hummel, 2008)

Other

Statement

Reference

Scer\GAL4^GMR.long, seq^UAS.cBa, sev³ has photoreceptor cell R7 phenotype

(Petrovic and Hummel, 2008)

Scer\GAL4^GMR.long, seq^UAS.cBa, sev³ has medulla layer M3 phenotype

(Petrovic and Hummel, 2008)

Scer\GAL4^GMR.long, seq^UAS.cBa, sev³ has medulla layer M6 phenotype

(Petrovic and Hummel, 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of seq^Scer\UAS.cBa and bnl^Scer\UAS.cSa in the CNS under the control of Scer\GAL4¹⁴⁰⁷ has the same effect as over-expression of bnl^Scer\UAS.cSa alone.

(Araújo and Casanova, 2011)

Expression of seq^Scer\UAS.cBa under the control of Scer\GAL4^GMR.long induces the R7 photoreceptor cells in sev³ mutants to switch from targeting the M3 layer of the medulla to the M6 layer.

CadN⁴⁰⁵ suppresses the R8 cell targeting defects seen when seq^Scer\UAS.cBa is expressed under the control of Scer\GAL4^GMR.long. The R8 axons no longer terminate in the M6 layer of the medulla and correctly target the M3 layer. However the flies do show an R7 targeting phenotype; the R7 photoreceptors target the M3 layer rather than the M6 layer, as is seen in CadN⁴⁰⁵ mutants alone.

(Petrovic and Hummel, 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

seq^UAS.cBa/Scer\GAL4^69B rescues seq^Z1241

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^69B rescues seq^C022/seq^Z1241

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^69B rescues seq^C022

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^GMR.long rescues seq^unspecified

(Petrovic and Hummel, 2008)

Partially rescues

seq^UAS.cBa/Scer\GAL4^sim.PS partially rescues seq^Z1241

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^sim.PS partially rescues seq^C022/seq^Z1241

(Araújo and Casanova, 2011)

seq^UAS.cBa/Scer\GAL4^sim.PS partially rescues seq^C022

(Araújo and Casanova, 2011)

Comments

Expression of seq^Scer\UAS.cBa in the ectoderm under the control of Scer\GAL4^69B rescues the ganglionic branch bifurcation phenotype found in seq^C022 and seq^Z1241 homozygotes and seq^Z1241/seq^C022 trans-heterozygotes.

Expression of seq^Scer\UAS.cBa in midline precursors and glia of seq^Z1241, seq^C022 and seq^Z1241/seq^C022 mutants under the control of Scer\GAL4^sim.PS accomplishes approximately 50% rescue with those embryos that were not fully rescued showing a considerable attenuation of the phenotype.

(Araújo and Casanova, 2011)

Expression of seq^Scer\UAS.cBa under the control of Scer\GAL4^GMR.long rescues the R7/R8 photoreceptor targeting defects seen when seq^unspecified mutant somatic clones are induced in R7 cells.

(Petrovic and Hummel, 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9244

w^*; P{UAS-seq.B}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

seq^Scer\UAS.cBa

seq^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Brenman

(Brenman, 2005.8)

Secondary FlyBase IDs

References (7)

Report Sections

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