FB2024_03 , released June 25, 2024
Allele: Dmel\spasUAS.cSa
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General Information
Symbol
Dmel\spasUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Sherwood
FlyBase ID
FBal0177735
Feature type
allele
Associated gene
Dmel\spas
Associated Insertion(s)
Carried in Construct
P{UAS-spas.S}
Also Known As
UAS-spastin
Key Links
Transgenic product class
cDNA
(Sherwood et al., 2004)
wild_type
(Sherwood et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Sherwood et al., 2004)
wild_type
(Sherwood et al., 2004)
Mutagen
in vitro construct
(Sherwood et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-spas.S}
Cytology
Description

UASt sequences drive expression of a 2.9kb fragment including a spas cDNA, and 350bp of 3' sequences (excluding 681bp of the 3' UTR).

(Sherwood et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spas
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of spasScer\UAS.cSa under the control of Scer\GAL4e22c almost completely eliminates microtubules, without causing substantial differences in cell shape.

      (Bulgakova et al., 2013)

      Overexpression of spasScer\UAS.cSa in class IV nociceptive neurons, under the control of Scer\GAL4ppk.1.9 results in a strong reduction in dendrite branch complexity. futsch-positive neuronal microtubules are reduced in these arbors compared to controls.

      (Stewart et al., 2012)

      Ectopic expression of spasScer\UAS.cSa in class I ddaE neurons under the control of Scer\GAL4221 results in disruption of arbor morphology.

      (Jinushi-Nakao et al., 2007)

      When spasScer\UAS.cSa is driven by Scer\GAL4sca-537.4, at 23oC, the ventral nerve cord (VNC) collapses onto the midline and discrete longitudinal bundles are no longer apparent. When spasScer\UAS.cSa is driven by Scer\GAL4spin.PN mutant animals do survive to the third instar. When spasScer\UAS.cSa is driven by Scer\GAL4G14 the microtubule network normally seen in muscles is erased.

      (Sherwood et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      spasScer\UAS.cSa
      spasUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Sherwood
      (Sherwood et al., 2004)
      Secondary FlyBase IDs
        References (8)