FB2024_03 , released June 25, 2024
Allele: Dmel\5-HT1AUAS.cRa
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General Information
Symbol
Dmel\5-HT1AUAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Rosay
FlyBase ID
FBal0176432
Feature type
allele
Associated gene
Dmel\5-HT1A
Associated Insertion(s)
Carried in Construct
P{UAS-5-HT1A.R}
Key Links
Transgenic product class
wild_type
(Kerr et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kerr et al., 2004)
Mutagen
in vitro construct
(Kerr et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-5-HT1A.R}
Cytology
Description

UAS regulatory sequences drive expression of 5-HT1A.

(Kerr et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
5-HT1A
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Pan-neuronal expression of 5-HT1AScer\UAS.cRa in adult flies (using 31[o]C heat-induced Scer\GAL4nSyb.PS in combination with Scer\GAL80ts.αTub84B) does not significantly affect wall following behavior in an arena (posited measure of anxiety) or walking speed, compared to non-induced (18[o]C) controls.

      (Mohammad et al., 2016)

      5HT stimulates fluid secretion in Malpighian tubules isolated from 5-HT1AScer\UAS.cRa; Scer\GAL4c42 or 5-HT1AScer\UAS.cRa; Scer\GAL4tsh-c724 flies. cAMP and CGMP levels are unaffected. The increased fluid scretion is inhibited by yohimbine.

      (Kerr et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/5-HT1AUAS.cRa rescues 5-HT1AΔ5kb

      (Yuan et al., 2006)
      Comments

      Expression of 5-HT1AScer\UAS.cRa pan-neuronally, under the control of Scer\GAL4elav-C155 restores sleep levels and consolidation in 5-HT1AΔ5kb mutants, with total sleep and night-time sleep bout length increasing while bout number decreases.

      Total daily sleep and night-time sleep bout length are restored to wild-type levels in RU486-treated 5-HT1AScer\UAS.cRa mutants, driven with Scer\GAL4Mef2.247.Switch (activated by RU486).

      (Yuan et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      P. Rosay

      (Kerr et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      5-HT1AScer\UAS.cRa
      5-HT1AUAS.cRa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of Rosay
      (Kerr et al., 2004)
      Secondary FlyBase IDs
        References (5)