FlyBase Allele Report: Dmel\tefu[stg]

General Information

Symbol

Dmel\tefu^stg

Species

D. melanogaster

Name

FlyBase ID

FBal0175409

Feature type

allele

Associated gene

Dmel\tefu

Associated Insertion(s)

TI{TI}tefu^stg

Carried in Construct

Also Known As

atm^stg

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Gong et al., 2005, Bi et al., 2004)

I-SceI

(Gong et al., 2005)

FLPase

(Gong et al., 2005)

ends-in gene targeting

(Bi et al., 2004)

gene targeting by homologous recombination

(Gong et al., 2005)

Progenitor genotype

P{FRT(tefu.Scer\SceI.RS.cGa)}

(Gong et al., 2005)

Associated Insertion(s)

TI{TI}tefu^stg

Cytology

Description

Duplication of the tefu locus. The 5' copy contains a +1 frameshift at codon 1187, which results in a stop codon 31 codons downstream. The 3' copy contains stop codons in all six reading frames at the position corresponding to codon 195. A w^+mW.hs marker and a single FRT site are located between the two copies of tefu.

(Gong et al., 2005)

Ends-in gene targeting event at the tefu locus.

(Bi et al., 2004)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome, telomeric region

(Bi et al., 2004)

chromosome, telomeric region | embryonic stage | maternal effect (with tefu^ZIII-5190)

(Morciano et al., 2013)

melanotic mass | third instar larval stage

(Wang et al., 2013)

mitosis & nuclear chromosome

(Bi et al., 2004)

Detailed Description

Statement

Reference

tefu^ZIII-5190/tefu^stg adults are recovered at a Mendelian ratio, indicating that the tefu^ZIII-5190 allele minimally affects viability.

tefu^ZIII-5190/tefu^stg females produce a normal amount of eggs. However, none of the embryos hatch, regardless of paternal genotype. The embryos show significant development, indicating that they are fertilised. Mitotic defects are seen in 0-2 hour embryos derived from tefu^ZIII-5190/tefu^stg females: chromosome bridges are seen between segregating nuclei, multilobed nuclei are seen and large areas of the surface of the embryo which are free of nuclei are observed. Metaphase chromosomes exhibit end-to-end telomere fusions.

(Morciano et al., 2013)

tefu^stg/tefu^stg third instar larvae have spontaneous melanotic tumors (around 1.2%).

(Wang et al., 2013)

Mutant larvae show a normal checkpoint response (a steep decline in the number of G₂ cells entering mitosis) in response to irradiation with 4000 rad of X rays, but they are unable to block mitotic entry when irradiated with 500 rad.

(Bi et al., 2005)

Dividing neuroblasts in the brains of third instar mutant larvae show a number of defects. The most prominent defect is end-to-end association (telomere attachment) of chromosomes. Single telomere attachments (both between sister chromatids and between non-sister chromatids) and double telomere attachments are seen. In addition, chromosome breaks and aneuploid nuclei are seen. Chromosome bridges are often seen.

(Bi et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

tefu^stg has melanotic mass phenotype | third instar larval stage phenotype, enhanceable by p53^5A-1-4/p53^5A-1-4

(Wang et al., 2013)

tefu^stg has melanotic mass phenotype | third instar larval stage phenotype, enhanceable by Clbn^1Q/Clbn^1Q

(Wang et al., 2013)

Other

Statement

Reference

mre11^Δ35K1, tefu^stg has lethal | recessive | larval stage phenotype

(Bi et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

tefu^stg has melanotic mass | third instar larval stage phenotype, enhanceable by p53^5A-1-4/p53^5A-1-4

(Wang et al., 2013)

tefu^stg has melanotic mass | third instar larval stage phenotype, enhanceable by Clbn^1Q/Clbn^1Q

(Wang et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Clbn^1Q/Clbn^1Q or p53^5A-1-4/p53^5A-1-4 significantly enhances melanotic tumor formation in tefu^stg/tefu^stg third instar larvae (36.3% and 29.5%, respectively).

(Wang et al., 2013)

tefu^stg p53^unspecified double mutants show the same checkpoint phenotype as tefu^stg single mutants; they show a normal checkpoint response to irradiation with 4000 rad of X rays, but they are unable to block mitotic entry when irradiated with 500 rad. mei-41^D9 ; tefu^stg and mei-41^RT1 ; tefu^stg double mutant larvae show complete loss of the checkpoint response when irradiated with either 500 or 4000 rad of X rays.

(Bi et al., 2005)

tefu^stg mre11^Δ35K1 double homozygotes die as late third instar larvae. Double mutants do not show an additive effect on the frequency of telomere attachments and chromosome breaks in third larval instar neuroblasts.

(Bi et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dp(3;3)tefu^stg

atm^stg

(Gao et al., 2010, Bi et al., 2004)

atm^strong

(Bi et al., 2004)

tefu^stg

(Morciano et al., 2013, Gong et al., 2005)

Name Synonyms

Secondary FlyBase IDs

FBab0046723

References (6)

Report Sections

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