FB2024_03 , released June 25, 2024
Allele: Dmel\aPKCM.UAS
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General Information
Symbol
Dmel\aPKCM.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0159524
Feature type
allele
Associated gene
Dmel\aPKC
Associated Insertion(s)
Carried in Construct
P{UAS-PKM}
Also Known As
UAS-PKM
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Ruiz-Canada et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-PKM}
Cytology
Description
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
aPKC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  carcinoma
      CEA
      (Eder et al., 2005)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When aPKCM.Scer\UAS is driven by Scer\GAL4C380 (presynaptically) or Scer\GAL4BG487 (postsynaptically) a reduction in the number of synaptic boutons is seen. Muscle size is unaffected. When aPKCM.Scer\UAS is driven by Scer\GAL4C380 (presynaptically) the microtubules in the terminal boutons are more numerous and 65% longer than in control NMJs. When aPKCM.Scer\UAS is driven by Scer\GAL4BG487 (postsynaptically) microtubules (MTs) surrounding synaptic boutons are significantly sparser than wild-type. In these larvae,, the Spectrin-rich area is expanded and is seen to fill much of the space devoid of MTs. The morphology of the synaptic boutons is also aberrant. The separation between individual boutons is often lost in these boutons. Animals expressing aPKCM.Scer\UAS driven by Scer\GAL4BG487 (postsynaptically) exhibit a substantial decrease in spontaneous miniature excitatory junctional potential (mEJP) amplitude. The frequency of mEJPs is decreased. The amplitude of evoked junctional potentials (EJPs) is decreased by 75%. The overall quantal content is dramatically reduced in mutants.

      (Ruiz-Canada et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4futsch-C380/aPKCM.UAS is a non-suppressor of bouton phenotype of futschK68

      (Ruiz-Canada et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      aPKCM.Scer\UAS
      aPKCM.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)