FlyBase Allele Report: Dmel\Atg8a[KG07569]

General Information

Symbol

Dmel\Atg8a^KG07569

Species

D. melanogaster

Name

FlyBase ID

FBal0157024

Feature type

allele

Associated gene

Dmel\Atg8a

Associated Insertion(s)

P{SUPor-P}Atg8a^KG07569

Carried in Construct

Also Known As

Atg8a²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(FlyBase, 1996-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Atg8a^KG07569

Cytology

Description

Insertion in codon 28 of the open reading frame.

(Scott et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:10,767,990..10,767,990 [+]

(Tan et al., 2024, Tsapras et al., 2022, Bourouis et al., 2019, Billes et al., 2018, Tusco et al., 2017, Menzies et al., 2015, Liu et al., 2013, Shravage et al., 2013, Pircs et al., 2012, Midorikawa et al., 2010, Nezis et al., 2008, Scott et al., 2007, FlyBase, 1996-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^GMR.Q120, CalpA^KK104532

(Menzies et al., 2015)

ameliorates sensory peripheral neuropathy

modeled by CCT5^KK108095

(Pavel et al., 2016)

modeled by CCT5^NIG.8439R

(Pavel et al., 2016)

modeled by CCT7^KK101540

(Pavel et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg8a^KG07569 homozygous adults are hypersensitive to acute food deprivation.

(Bourouis et al., 2019)

Atg8a^KG07569 adults are much more sensitive to paraquat-induced oxidative stress than controls.

(Robin et al., 2019)

Atg8a^KG07569 homozygous adult midguts do not show significant changes in the number of pre-enteroendocrine cells, as compared to heterozygous or wild-type controls.

(Nagy et al., 2017)

Atg8a^KG07569 mutant third instar larvae display highly increased number of lipid droplets in prothoracic gland cells.

(Danielsen et al., 2016)

Atg8a^KG07569 mutants display significantly delayed midgut degradation after pupariation, with significantly increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.

(Xu et al., 2015)

Autophagy is significantly decreased in Atg8a^KG07569 mutant fat body cells.

(Liu et al., 2013)

18% of Atg8a^KG07569 mutant pupae contain melanotic masses, such cell masses are not observed in Atg8a^KG07569 mutant larvae.

(Shravage et al., 2013)

Salivary glands appear normal in 6 hour mutant prepupae. However, at 24 hours after puparium formation, the mutant animals still contain salivary gland tissue (containing vacuolated salivary gland fragments), in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.

DNA fragmentation is detected by TUNEL in salivary glands of Atg8a^KG07569 animals at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage.

(Berry and Baehrecke, 2007)

Starvation-induced autophagy is significantly reduced in mutant animals.

(Scott et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Atg8a^KG07569, Scer\GAL4^Act5C.PP is a suppressor of increased cell death | somatic clone phenotype of Atg1^UAS.cSa, Scer\GAL4^Act5C.PP

(Scott et al., 2007)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is a non-suppressor of visible phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is a non-suppressor of abnormal size phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is an enhancer of rhabdomere | progressive phenotype of Hsap\HTT^GMR.Q120, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

NOT Suppressor of

Statement

Reference

Dcr-2^UAS.cDa, CalpA^KK104532, Atg8a^KG07569, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\HTT^{Q46.ex1p.UAS.EGFP}, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is a non-suppressor of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The induction of autophagy caused by expression of Atg1^Scer\UAS.cSa under the control of Scer\GAL4^hs.PH is significantly suppressed by Atg8a^KG07569.

The elimination of cells expressing Atg1^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP from the wing disc is significantly reduced in a Atg8a^KG07569 background.

(Scott et al., 2007)

Xenogenetic Interactions

Statement

Reference

Co-expression of CalpA^KK104532 in an Atg8a^KG07569 mutant genetic background fails to reduce the number of aggregates observed in the eyes of flies expressing Scer\GAL4^GMR.PF>Hsap\HTT^{Q46.ex1p.Scer\UAS.T:Avic\GFP-EGFP}.

Co-expression of CalpA^KK104532 in an Atg8a^KG07569 mutant genetic background exacerbates the toxicity of Hsap\HTT^GMR.Q120 expression in the eye.

Co-expression of CalpA^KK104532 in an Atg8a^KG07569 mutant genetic background fails to suppress the toxicity of Scer\GAL4^GMR.PF>Hsap\MAPT^Scer\UAS.cWa.

(Menzies et al., 2015)

Complementation and Rescue Data

Rescued by

Atg8a^KG07569 is rescued by Atg8a^3x.mCherry

(Tan et al., 2024)

Atg8a^KG07569 is rescued by Atg8a^hs.GFP

(Berry and Baehrecke, 2007)

Atg8a^KG07569 is rescued by Atg8a^hs.GFP

(Scott et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

14639

y¹ P{SUPor-P}Atg8a^KG07569/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Atg8a²

(Bourouis et al., 2019, Ratliff et al., 2016, Bartlett et al., 2011)

Atg8a^KG07659

(Menzies et al., 2015)

Atg8a^KG

(Tusco et al., 2017)

CG32672^KG07569

atg8^KQ70569

(Fouillet et al., 2012)

atg8a^KG07569

(Robin et al., 2019, Berry and Baehrecke, 2007)

Name Synonyms

Secondary FlyBase IDs

References (36)

Report Sections

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