FlyBase Allele Report: Dmel\hpo[42-47]

General Information

Symbol

Dmel\hpo^42-47

Species

D. melanogaster

Name

FlyBase ID

FBal0151857

Feature type

allele

Associated gene

Dmel\hpo

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Willecke et al., 2006)

Mutagen

X ray

(Wu et al., 2003)

Progenitor genotype

Cytology

Description

An 18bp deletion in thee hpo ORF, leading to an in frame deletion of residues N166-T171 inclusive, in the predicted kinase domain.

(Wu et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Hamaratoglu et al., 2006)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates gastrointestinal adenoma

modeled by Apc^Q8, Apc2^g10

(Suijkerbuijk et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

hpo^42-47/+ third instar larvae display normal number of satellite boutons on neuromuscular junctions.

(Sakuma et al., 2016)

hpo^42-47/+ does not have any affect on cell growth or proliferation in the adult posterior midgut, as compared to controls.

(Suijkerbuijk et al., 2016)

Approximately 60% of hpo^42-47 mutant border cell clusters are delayed at stage 10 of oogenesis. Unlike control clusters, F-actin fails to polarize to the outer rim of hpo^42-47 mutant clusters and instead tends to accumulate throughout the cluster. Border cell specification is not affected in these mutants.

(Lucas et al., 2013)

hpo^42-47 mutants display a small wing phenotype.

(Yin et al., 2013)

Adults containing homozygous clones represent only 2.1% of the population recovered after clones are induced using the eyFLP method (expected fraction of adults containing mutant clones is 50% if there is no effect of the mutant clones on viability).

(Genevet et al., 2009)

hpo^42-47 homozygous mutant clones generated in the male or female germline develop normally. In the testis 16 cells are observed per cyst, and cell size and morphology are indistinguishable from neighbouring control cells.

(Sun et al., 2008)

Eyes composed almost entirely of hpo^42-47 mutant tissue are overgrown and noticeably larger than control eyes.

(Pellock et al., 2007)

Apoptosis is reduced by up to 3-fold in hpo^42-47 clones of wing or eye discs in response to γ-rays compared to wild-type tissue.

(Colombani et al., 2006)

The mid-pupal retina of hpo^42-47/hpo^42-47 animals contains a large excess of inter-ommatidial cells. The resulting adult eyes are distorted and lumpy.

(Hamaratoglu et al., 2006)

Animals bearing hpo^42-47 clones show an overgrowth phenotype in the head.

(Polesello et al., 2006)

When somatic clones of hpo^42-47 homozygous cells are generated throughout the eye disc using Scer\FLP1^ey.PN with P{neoFRT}42D, only 2% of animals survive the pupal stage and eclose. The resulting flies have enlarged, folded eyes, and excess head cuticle. The external ommatidial facets are frequently lost. Sectioning of these eyes reveals a normal complement of photoreceptor cells. However, spacing between photoreceptor clusters is increased due to the presence of extra interommatidial pigment cells. In somatic clones of hpo^42-47 homozygous cells in the late third instar eye disc, ommatidial clusters have the normal complement of differentiating photoreceptor cells, and R8 is specified at correct location and density emerging from the morphogenetic furrow. The spacing between adjacent ommatidial clusters is initially normal but increases at later stages (more posterior to the furrow), due to the presence of extra interommatidial cells. These extra cells seem to be due to a failure of uncommitted interommatidial cells to undergo cell cycle arrest posterior to the second mitotic wave. At least some of these cells continue to proliferate during early pupal development, as revealed by ectopic BrdU incorporation at 16 hr after puparium formation (APF). However, ectopic cell proliferation is undetectable beyond 24 hr APF. At 36 hr APF, cell death is suppressed in mutant clones, even though abundant apoptosis is detected in the neighboring wild-type cells. Cell death in hpo mutant clones is not simply delayed, since it could not be detected in mutant clones up to 48 hr APF. Somatic clones of hpo^42-47 homozygous cells in the adult cuticle are characterised by extensive tissue overgrowth and abnormally (roughly) textured cuticle in which cell-cell boundaries are apparent. The cells of hpo^42-47 homozygous somatic clones in wing discs during the growth phase have a doubling time of 12.2 hours, compared to 13.9 hours for wild-type cells. This is due to a proportional acceleration of all phases of the cell cycle, rather than an effect in any specific stage.

(Wu et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hpo^42-47 has abnormal cell migration phenotype, suppressible by ena²¹⁰

(Lucas et al., 2013)

Suppressor of

Statement

Reference

hpo^42-47/hpo[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9

(Sakuma et al., 2016)

hpo^42-47/hpo[+] is a suppressor of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

hpo^42-47/hpo[+] is a suppressor of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

hpo^42-47 is a suppressor | partially of increased cell death phenotype of p53^GMR.Ex

(Colombani et al., 2006)

hpo^42-47/hpo^42-47 is a suppressor | partially of increased cell death | third instar larval stage phenotype of Scer\GAL4^GMR.PF, ex^UAS.cBa

(Hamaratoglu et al., 2006)

hpo^42-47/hpo[+] is a suppressor of abnormal size | adult stage phenotype of CycE^AR95/CycE^JP

(Wu et al., 2003)

NOT Suppressor of

Statement

Reference

hpo^42-47/hpo[+] is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9

(Sakuma et al., 2016)

Other

Statement

Reference

Scer\GAL4^Tub.PU, Scer\GAL80^αTub84B.PL, hpo^42-47, wts^{T1077A.UAS.Tag:Myr(Src64B),Tag:V5} has decreased cell size | somatic clone phenotype

(Yin et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

hpo^42-47 has border follicle cell phenotype, suppressible by ena²¹⁰

(Lucas et al., 2013)

hpo^42-47 has wing phenotype, suppressible by wts^{T1077A.UAS.Tag:Myr(Src64B),Tag:V5}/Scer\GAL4^nub.PU

(Yin et al., 2013)

hpo^42-47 has eye | somatic clone phenotype, suppressible by Hsap\STK3^hs.PW

(Wu et al., 2003)

hpo^42-47 has adult external head | somatic clone phenotype, suppressible by Hsap\STK3^hs.PW

(Wu et al., 2003)

hpo^42-47 has ommatidium | somatic clone phenotype, suppressible by Hsap\STK3^hs.PW

(Wu et al., 2003)

Suppressor of

Statement

Reference

hpo^42-47/hpo[+] is a suppressor of NMJ bouton | increased number phenotype of Scer\GAL4^RapGAP1-OK6, Strip^RNAi.UAS.9

(Sakuma et al., 2016)

hpo^42-47/hpo[+] is a suppressor of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

hpo^42-47/hpo[+] is a suppressor of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

hpo^42-47/hpo^42-47 is a suppressor of interommatidial cell | pupal stage phenotype of Scer\GAL4^GMR.PF, ex^UAS.cBa

(Hamaratoglu et al., 2006)

hpo^42-47/hpo^42-47 is a suppressor | partially of eye disc | third instar larval stage phenotype of Scer\GAL4^GMR.PF, ex^UAS.cBa

(Hamaratoglu et al., 2006)

hpo^42-47/hpo[+] is a suppressor | partially of ommatidium phenotype of CycE^AR95/CycE^JP

(Wu et al., 2003)

hpo^42-47/hpo[+] is a suppressor | partially of eye phenotype of CycE^AR95/CycE^JP

(Wu et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of satellite boutons on neuromuscular junctions in third instar larvae expressing Strip^{dsRNA.shRNA.Scer\UAS.9} under the control of Scer\GAL4^RapGAP1-OK6 is rescued by combination with single copy of hpo^42-47, whereas the increased frequency of miniature excitatory junction potentials characteristic remains high.

(Sakuma et al., 2016)

hpo^42-47/+ fully suppresses the reduced growth seen in posterior midgut wild type clones when in the presence of Apc^Q8/Apc^Q8, Apc2^g10/Apc2^g10 mutant clones, and suppresses the growth of Apc^Q8/Apc^Q8, Apc2^g10/Apc2^g10 mutant clones.

(Suijkerbuijk et al., 2016)

Expression of hppy^Scer\UAS.cZa under the control of Scer\GAL4^tub.PU suppresses growth in hpo^42-47 mutant wing disc clones.

Expression of hppy^Scer\UAS.cZa under the control of Scer\GAL4^tub.PU does not suppress growth in wts^x1 mutant wing disc clones.

hppy^Δ1 does not increase the size of hpo^42-47 mutant wing disc clones.

(Zheng et al., 2015)

The border cell migration phenotype found in hpo^42-47 mutants is suppressed by a ena²¹⁰ background. These double mutants exhibit a normally polarised actin cytoskeleton.

(Lucas et al., 2013)

Expression of wts^{T1077A.Scer\UAS.T:Myr-Src64B,T:SV5\V5} under the control of Scer\GAL4^nub.PU suppresses the small wing phenotype found in hpo^42-47 mutants. hpo^42-47 mutant clones expressing wts^{T1077A.Scer\UAS.T:Myr-Src64B,T:SV5\V5} are smaller in size than those not expressing wts^{T1077A.Scer\UAS.T:Myr-Src64B,T:SV5\V5}.

(Yin et al., 2013)

he loss of inter-ommatidial cells in the developing retinas of ex^Scer\UAS.cBa; Scer\GAL4^GMR.PF animals at the mid-pupal stage is completely suppressed by hpo^42-47/hpo^42-47. The resulting retinas have an excess of inter-ommatidial cells, just as hpo^42-47/hpo^42-47 animals so.

(Hamaratoglu et al., 2006)

The head overgrowth phenotype of flies carrying hpo^42-47 clones is not significantly enhanced in a Rassf^X36 background.

(Polesello et al., 2006)

The small, rough eye phenotype of CycE^AR95/CycE^JP animals, is almost completely dominantly suppressed by hpo^42-47.

(Wu et al., 2003)

Xenogenetic Interactions

Statement

Reference

The severe eye and heat cuticle deformities seen in adults carrying somatic clones of hpo^42-47 homozygous cells throughout the eye disc (generated using Scer\FLP1^ey.PN with P{neoFRT}42D) are almost completely suppressed by Hsap\STK3^hs.PW, given a suitable heat shock regime (60 minutes daily from 2nd instar until eclosion).

(Wu et al., 2003)

Complementation and Rescue Data

Rescued by

hpo^42-47 is rescued by hpo^+t4.0

(Wu et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (32)

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