FB2024_03 , released June 25, 2024
Allele: Dmel\parkRNAi.UAS
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General Information
Symbol
Dmel\parkRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0151644
Feature type
allele
Associated gene
Dmel\park
Associated Insertion(s)
Carried in Construct
P{UAS-park.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Yang et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Yang et al., 2003)
Mutagen
in vitro construct
(Yang et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-park.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of park sequences which are arranged in an inverted repeat. This construct should produce park dsRNA.

(Yang et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
park
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Parkinson's disease
      CEA
      (M'Angale and Staveley, 2017)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Parkinson's disease
      modeled by Hsap\GPR37UAS.cYa
      (Yang et al., 2003)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of parkdsRNA.UAS under the control of Scer\GAL4Ddc.PL results in decreased lifespan and severely impaired adult locomotion, Scer\GAL4GMR.PF-driven expression leads to reduced number of ommatidia in adult eyes and disrupted ommatidial lattice.

      (M'Angale and Staveley, 2017)

      Whole-eye knockdown of park, through expression of parkdsRNA.Scer\UAS under the control of Scer\GAL4ey.PH leads to lipid droplet accumulation. Aconitase activity is reduced to less than 50% of wild-type in these mutants and there is a strong correlation between decreased aconitase enzymatic activity and the number of lipid droplets per ommatidium, suggesting reactive oxygen species may play a role in lipid droplet accumulation.

      (Liu et al., 2015)

      Expression of parkdsRNA.Scer\UAS under the control of Scer\GAL4Ddc.PL does not significantly affect the number of dopaminergic neurons in the dorsomedial clusters of the brain in 30 day old flies.

      (Yang et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduced adult lifespan along with the impaired climbing ability characteristic for flies expressing parkdsRNA.UAS under the control of Scer\GAL4Ddc.PL is significantly enhanced by co-expression of BuffyUAS.cQa. In contrast, the disrupted ommatidial lattice and loss of ommatidia of flies expressing parkdsRNA.UAS driven by Scer\GAL4GMR.PF is ameliorated by BuffyUAS.cQa.

      (M'Angale and Staveley, 2017)
      Xenogenetic Interactions
      Statement
      Reference

      Coexpression of parkdsRNA.Scer\UAS results in a dosage-dependent acceleration of the dopaminergic (DA) neuron degeneration that is seen in flies expressing Hsap\GPR37Scer\UAS.cYa under the control of Scer\GAL4Ddc.PL; flies co-expressing Hsap\GPR37Scer\UAS.cYa and two copies of parkdsRNA.Scer\UAS show the degeneration phenotype at 14 days of age, whereas flies expressing Hsap\GPR37Scer\UAS.cYa alone or Hsap\GPR37Scer\UAS.cYa and one copy of parkdsRNA.Scer\UAS have a relatively normal number of DA neurons in the dorsomedial clusters. The severity of the DA neuron degeneration phenotype is also enhanced.

      (Yang et al., 2003)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      parkRNAi.UAS
      parkdsRNA.Scer\UAS
      parkdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)