FlyBase Allele Report: Dmel\slif[UY681]

General Information

Symbol

Dmel\slif^UY681

Species

D. melanogaster

Name

FlyBase ID

FBal0151480

Feature type

allele

Associated gene

Dmel\slif

Associated Insertion(s)

P{Mae-UAS.6.11}slif^UY681

Carried in Construct

Also Known As

slif^Anti, UAS-Slif-anti

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Colombani et al., 2003, Raisin et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{Mae-UAS.6.11}slif^UY681

Cytology

Description

The P{Mae-UAS.6.11} is inserted in the first intron of the slif gene. The orientation of the insert is opposite to the direction of transcription.

(Colombani et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{Mae-UAS.6.11}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by dlg1^GD4689, Ras85D^V12.UAS

(Katheder et al., 2017)

modeled by Raf^F22.hs

(Zhao et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | larval stage, with Scer\GAL4^ple.TH-D'

(Bjordal et al., 2014)

abnormal developmental rate, with Scer\GAL4^ppl.PP

(Colombani et al., 2003)

abnormal feeding behavior | adult stage | female, with Scer\GAL4^fit.PS

(Sun et al., 2017)

abnormal feeding behavior | larval stage, with Scer\GAL4^ple.PF

(Bjordal et al., 2014)

abnormal feeding behavior | larval stage, with Scer\GAL4^ple.TH-D'

(Bjordal et al., 2014)

decreased body size | pupal stage, with Scer\GAL4^Ilp2.PR

(Pathak et al., 2021)

decreased cell number, with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

decreased cell number (with Df(3L)Δ1AK), with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

decreased cell size, with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

decreased cell size, with Scer\GAL4^ppl.PP

(Colombani et al., 2003)

decreased cell size (with Df(3L)Δ1AK), with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

decreased occurrence of cell division | larval stage, with Scer\GAL4^Cg.PA

(Sousa-Nunes et al., 2011)

lethal - all die before end of larval stage | partially, with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

some die during larval stage | partially, with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

Phenotype Manifest In

embryonic/larval fat body, with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

embryonic/larval fat body, with Scer\GAL4^ppl.PP

(Colombani et al., 2003)

larva, with Scer\GAL4^ppl.PP

(Colombani et al., 2003)

larval neuroblast, with Scer\GAL4^Cg.PA

(Sousa-Nunes et al., 2011)

lipid droplet & larval fat body, with Scer\GAL4^ppl.PP

(Gutierrez et al., 2007)

lipid droplet & larval oenocyte, with Scer\GAL4^Lsp2.PH

(Gutierrez et al., 2007)

lipid droplet & larval oenocyte, with Scer\GAL4^ppl.PP

(Gutierrez et al., 2007)

wing, with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

wing (with Df(3L)Δ1AK), with Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Raisin et al., 2003)

Detailed Description

Statement

Reference

Adult females expressing slif^UY681 under the control of Scer\GAL4^fit.PS show significantly weaker suppression of feeding (on standard food) after pre-feeding with tryptone compared to controls.

(Sun et al., 2017)

Expression of slif^UY681, throughout development, under the control of Scer\GAL4^ple.PF, but not Scer\GAL4^NPF.PU, Scer\GAL4^Trh.PB, Scer\GAL4^Tdc2.PU, Scer\GAL4^ChAT.PU, Scer\GAL4^Gad1.3.098, Scer\GAL4^Hug.PU or Scer\GAL4^386Y, results in a significant reduction in larval food intake, compared to controls; this effect also occurs when a Gal80[ts] transgene is used to restrict expression under the control of Scer\GAL4^ple.PF to the 16 hours prior to analysis.

Expression of slif^UY681 under the control of Scer\GAL4^ple.TH-D', but not Scer\GAL4^ple.TH-C, in combination with a Gal80[ts] transgene to restrict expression to the 16 hours prior to analysis, reduces larval food intake and body size, compared to controls.

(Bjordal et al., 2014)

Central nervous system neuroblasts of fed larvae expressing slif^UY681 under the control of Scer\GAL4^Cg.PA show a reduction in EdU incorporation compared to controls.

(Sousa-Nunes et al., 2011)

Larvae expressing slif^UY681 under the control of Scer\GAL4^ppl.PP and raised to the third instar on a standard diet resemble starved wild-type larvae in that lipid droplets aggregate in the fat body. Oenocytes in third instar larvae expressing slif^UY681 under the control of Scer\GAL4^ppl.PP contain numerous lipid droplets, both in larvae that have been fed on a standard diet or those that have been food-deprived for 14 hours (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).

Expression of slif^UY681 under the control of Scer\GAL4^Lsp2.PH results in an accumulation of lipid droplets in the oenocytes of fed larvae.

(Gutierrez et al., 2007)

Colored food is present in the gut of Scer\GAL4^ppl.PP>slif^UY681 animals, showing that these mutants are able to swallow and process food.

(Colombani et al., 2003)

When slif^UY681 is driven by Scer\GAL4^Bx-MS1096 a reduction in size is seen. The animals have phenotypes mimicking amino acid deprivation, exhibiting storage vesicle aggregation in the fat body. When slif^UY681 is driven by Scer\GAL4^ppl.PP in the fat bodies, despite normal feeding behaviour and processing of food along the digestive tract, larval development is delayed and accompanied by significant growth defects. Growth rate is strongly reduced throughout larval life. When induced at 18^oC emerging adults are reduced in size and body weight (54% of wild-type). Stronger induction (at 25^oC) provokes a longer developmental delay associated with pupal lethality. Unlike for wild-type flies, a 90% reduction of arginine concentration in the food leads to a dramatic aggravation of the growth defect, with most animals dying as small L2 larvae. This aggravation is not seen after starvation for valine. growth in the salivary glands (a larval endoreduplicating tissue) are less affected than growth in imaginal discs. These larvae also displayed remobilisation of lipid vesicles in fat body cells, as well as a reduction in cell size and endoreduplication levels comparable to the effect of a diet lacking protein.

(Colombani et al., 2003)

Flies expressing slif^UY681 under the control of Scer\GAL4^en-e16E show a significant difference in growth between the posterior compartment of the wing (which expresses Scer\GAL4^en-e16E) and the anterior compartment of the wing (internal control).

(Raisin et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Pten¹¹⁷, Scer\GAL4^Act5C.PP, slif^UY681 has increased cell death | somatic clone phenotype, non-enhanceable by Atg5^RNAi.UAS, Scer\GAL4^Act5C.PP

(Nowak et al., 2013)

Pten¹¹⁷, Scer\GAL4^Act5C.PP, slif^UY681 has increased cell growth rate | somatic clone phenotype, non-enhanceable by Atg5^RNAi.UAS, Scer\GAL4^Act5C.PP

(Nowak et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^ple.PF, slif^UY681 has abnormal feeding behavior | larval stage phenotype, suppressible | partially by Gcn2^RNAi.UAS.cUa, Scer\GAL4^ple.PF

(Bjordal et al., 2014)

Enhancer of

Statement

Reference

slif^UY681/Scer\GAL4^Act5C.PP is an enhancer of increased cell death | somatic clone phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Suppressor of

Statement

Reference

slif^UY681, Scer\GAL4^{Act5C.CoinFLP-FRT} is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689

(Katheder et al., 2017)

slif^UY681, Scer\GAL4^{Act5C.CoinFLP-FRT} is a suppressor | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689

(Katheder et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

slif^UY681, Scer\GAL4^{Act5C.CoinFLP-FRT} is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^{Act5C.CoinFLP-FRT}, dlg1^GD4689

(Katheder et al., 2017)

slif^UY681/Scer\GAL4^Act5C.PP is a suppressor of eye | somatic clone phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larval eye-antennal imaginal disc clones co-expressing dlg1^GD4689 and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^{Act5C.CoinFLP-FRT}, in combination with Dicer-2 for efficient RNAi, form invasive tumors, whose size is significantly reduced by the additional clonal co-expression of slif^UY681.

(Katheder et al., 2017)

Expression of slif^UY681 in Pten¹¹⁷ mutant clones results in a slight reduction of the mutant tissue under normal conditions. Under starvation conditions (10g/l yeast), these double mutant cells are almost completely eliminated. This loss of Pten¹¹⁷ mutant tissue is accompanied by a massive increase in apoptosis. The structure of adult eyes in these mutants are wild-type, indicating that the surrounding tissue is able to compensate for the loss of the clones.

Knockdown of Atg5, through expression of Atg5^{dsRNA.Scer\UAS}, does not impact on the initial growth of Pten¹¹⁷ clones with reduced slif function (through expression of slif^UY681).

(Nowak et al., 2013)

Xenogenetic Interactions

Statement

Reference

Blocking apoptosis, through expression of BacA\p35^Scer\UAS.cHa in Pten¹¹⁷ clones with diminished slif function (through expression of slif^UY681), delays the collapse of the clones but they are absent from adult eyes.

(Nowak et al., 2013)

Complementation and Rescue Data

Partially rescued by

slif^UY681 is partially rescued by slif^UAS.cCa/Scer\GAL4^Bx-MS1096

(Colombani et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

52655

y¹ w^*; P{Mae-UAS.6.11}slif^UY681

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

UY681

(Colombani et al., 2003)

slif^ANTI

(Sousa-Nunes et al., 2011)

slif^Anti

(Barrio et al., 2014, Gutierrez et al., 2007, Colombani et al., 2003, Colombani et al., 2003)

slif^UY681

slif^anti

(Zhao et al., 2021, Nowak et al., 2013)

slifA

(Bjordal et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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