Female flies mated with SP⁰/Df(3L)Δ130 males display shorter mating refractory periods after copulation than females mated with control males.

The electrophysiological response of sex peptide abdominal ganglion (SAG) neurons following light-activation of sex peptide sensory neurons (SPSN) in SP⁰ females that have been mated with wild type males is indistinguishable from controls. Many wild type females that have been mated with SP⁰ males show an impaired response, although some still have a physiology similar to that of females mated to SP[+] males. Females that have been mated to SP⁰ males show increased receptivity compared to controls, but this is still significantly lower than in virgin flies. SP⁰-mated females that actively reject males post-mating tend to also exhibit an impaired SAG-neuron response.

Females mated with SP⁰ mutant males behave very much like virgins.

Females mated to Acp70A⁰/Df(3L)Δ130 males show high re-mating activity (comparable to those of virgin female controls) at 48 hours after the first mating.

Acp70A⁰/Df(3L)Δ130 males court females marginally significantly more than Acp70A[+] males.

Wild-type female flies mated to Acp70A⁰/Df(3L)Δ130 mutant males exhibit a severe reduction in egg-laying per female 48 hours after mating. Approximately 80% of these females copulate again within 1 hour after initial mating and appear receptive to mating.

Acp70A⁰ heterozygous males elicit a robust post-mating response from wild-type females.

Females previously mated to Acp70A⁰/Df(3L)Δ130 males remate more frequently and sooner than do females mated to control males.

Females mated to Acp70A⁰/Df(3L)Δ130 males lay significantly fewer eggs in the period between their first and second matings than females mated to control males.

Females mated to Acp70A⁰/Df(3L)Δ130 males do not show a reduction in receptivity to courting males and do not increase their egg-laying rate, in contrast to wild-type mated females.

Wild-type virgin females show the same receptivity towards a naive Acp70A⁰/Df(3L)Δ130 male as control females. However, these females lay very few eggs after mating, in contrast to control females. If the females that were mated to Acp70A⁰/Df(3L)Δ130 males are then tested for receptivity towards a second naive male after being allowed to lay eggs for 48 hours, they mate again at a high frequency and do not actively reject the second male, in contrast to control females tested under the same conditions.

Acp70A⁰ males fail to significantly induce feeding in females after mating. They exhibit courtship amd mating rates similar to those of controls and successfully fertilise females.

Females mated to Acp70A⁰ males exhibit only short term post-mating responses (PMR, e.g. increase in egg laying and reduction in receptivity). After two days the PMR are lacking, while in wild-type controls PMRs last for over 5 days.

Females mated with Acp70A⁰/Df(3L)Δ130 males show only a slight increase in oviposition on day 1 after mating and on the following days, the oviposition rate does not differ from the rate of unmated females (in contrast to females mated to wild-type males which show a considerable increase in oviposition in the first 4 days after mating). Females mated to Acp70A⁰/Df(3L)Δ130 males show a strong reduction of receptivity only 4 hours after copulation. 12 and 24 hours after copulation, receptivity is more than 60%. After 48 hours, receptivity has increased to virgin levels. Sperm of Acp70A⁰/Df(3L)Δ130 males are motile and are transferred and stored correctly.

SP⁰ has abnormal mating behavior | mated female limited phenotype, non-suppressible by Scer\GAL4^repo/moody^GD709

SP⁰ has abnormal mating behavior | mated female limited phenotype, non-suppressible by moody^KK100674/Scer\GAL4^repo

SP⁰ has abnormal mating behavior | mated female limited phenotype, non-suppressible by Df(1)JC70/Scer\GAL4^repo/Df(1)Exel6234/moody^GD709

SP⁰ has abnormal mating behavior | mated female limited phenotype, non-suppressible by Df(1)JC70/moody^KK100674/Scer\GAL4^repo/Df(1)Exel6234