FB2024_03 , released June 25, 2024
Allele: Dmel\Act5Cslbo.EGFP
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General Information
Symbol
Dmel\Act5Cslbo.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0150991
Feature type
allele
Associated gene
Dmel\Act5C
Associated Insertion(s)
Carried in Construct
P{slbo-EGFP::Act5C}
Key Links
Transgenic product class
wild_type
(Fulga and Rorth, 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Fulga and Rorth, 2002)
Mutagen
in vitro construct
(Fulga and Rorth, 2002)
Progenitor genotype
Carried in construct
P{slbo-EGFP::Act5C}
Cytology
Description

Expression of Act5C fused at the N-terminus to EGFP is under the control of 2 copies of a 2.6kb slbo enhancer fragment fused to Hsp70 minimal promoter sequences.

(Fulga and Rorth, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
slbo
Encoded product / tool
Act5C
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      border follicle cell & filopodium

      (Fulga and Rorth, 2002)
      Detailed Description
      Statement
      Reference

      In Act5Cslbo.T:Avic\GFP-EGFP (2 copies of 'strong' insertion P{slbo-EGFP::Act5C}2) animals the initiation of border follicle cell migration is inhibited. Arrested or delayed border cell clusters initially form normal looking cellular extensions, but these continue to grow as the border cell bodies fail to move. The extensions become very long and cease to be straight (as wild-type are), but continue to extend in the correct direction. These extra long cellular extensions sometimes fragment. Occasionally, a second border cell forms a second long cellular extension that follows a separate path.

      (Fulga and Rorth, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Act5Cslbo.T:Avic\GFP-EGFP and of psidinScer\UAS.cKa under the control of Scer\GAL4slbo.2.6 results in a strong inhibition of border follicle cell migration.

      (Kim et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Act5Cslbo.EGFP
      Act5Cslbo.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)