FB2024_03 , released June 25, 2024
Allele: Dmel\gnuUAS.EGFP
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General Information
Symbol
Dmel\gnuUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0150476
Feature type
allele
Associated gene
Dmel\gnu
Associated Insertion(s)
Carried in Construct
P{UAS-gnu-GFP.R}
Key Links
Transgenic product class
wild_type
(Renault et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Renault et al., 2003)
Mutagen
in vitro construct
(Renault et al., 2003)
Progenitor genotype
gnuEGFP
(Renault et al., 2003)
Carried in construct
P{UAS-gnu-GFP.R}
Cytology
Description

UAS sequences have been inserted to drive expression of gnu tagged with EGFP.

(Renault et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
gnu
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When gnuScer\UAS.T:Avic\GFP-EGFP is driven by Scer\GAL4mat.αTub67C.T:Hsim\VP16, egg chambers up to stages 8-10 have wild-type morphology. However subsequent stages are characterised by large amounts of irregularly localised, often fragmented, chromatin resulting from the degeneration of nurse cell nuclei. Actin organisation within the egg chambers are also abnormal. Defects are first seen at stage 10, when nurse cells fail to assemble the actin meshwork and do not dump their cytoplasmic contents into the oocyte.

      (Renault et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4VP16.mat.αTub67C, gnuUAS.EGFP has nurse cell & nucleus phenotype, suppressible by plu6

      (Renault et al., 2003)

      Scer\GAL4VP16.mat.αTub67C, gnuUAS.EGFP has nurse cell & nucleus phenotype, suppressible by png1058

      (Renault et al., 2003)
      NOT Enhancer of
      Statement
      Reference

      gnuUAS.EGFP/Scer\GAL4VP16.mat.αTub67C is a non-enhancer of egg & nucleus phenotype of plu6

      (Renault et al., 2003)

      gnuUAS.EGFP/Scer\GAL4VP16.mat.αTub67C is a non-enhancer of egg & nucleus phenotype of png1058

      (Renault et al., 2003)
      NOT Suppressor of
      Statement
      Reference

      gnuUAS.EGFP/Scer\GAL4VP16.mat.αTub67C is a non-suppressor of egg & nucleus phenotype of plu6

      (Renault et al., 2003)

      gnuUAS.EGFP/Scer\GAL4VP16.mat.αTub67C is a non-suppressor of egg & nucleus phenotype of png1058

      (Renault et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The addition of homozygous png5 to gnuScer\UAS.T:Avic\GFP-EGFP, Scer\GAL4mat.αTub67C.T:Hsim\VP16 animals completely restores ovarian function, though the eggs don't hatch and the png5 giant nuclei phenotype remains.

      (Renault et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      gnuScer\UAS.T:Avic\GFP-EGFP
      gnuUAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)