FlyBase Allele Report: Dmel\mir-ban[D.UAS.EGFP]

General Information

Symbol

Dmel\mir-ban^D.UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0147278

Feature type

allele

Associated gene

Dmel\mir-ban

Associated Insertion(s)

Carried in Construct

P{UAS-EGFP-mir-ban.D}

Also Known As

UAS-banD

Key Links

Transgenic product class

wild_type

(Brennecke et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brennecke et al., 2003)

Mutagen

in vitro construct

(Brennecke et al., 2003)

Progenitor genotype

Avic\GFP^EGFP.cSa.UAS

(Brennecke et al., 2003)

Carried in construct

P{UAS-EGFP-mir-ban.D}

Cytology

Description

Consists of a 100nt fragment that includes the hairpin region of the ban gene that has been cloned into the 3' UTR of Avic\GFP^EGFP.UAS.

(Brennecke et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-ban

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of ban^D.UAS.EGFP under the triple control of Scer\GAL4^VP16.nos.UTR, Scer\GAL4^nos.PG and Scer\GAL4^VP16.otu leads to a significant increase in germline stem cells per germarium, as compared to controls.

(Osman and Pek, 2018)

Expression of ban^{D.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^insc-Mz1407 in larval brains leads to an increase in the size of secondary neuroblasts and an increase in the nucleolar size in secondary neuroblasts, but no obvious change in the size of type II neuroblasts or type II neuroblast nucleoli, as compared to controls.

(Wu et al., 2017)

Expression of ban^{D.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^C855a results in overproliferation of the optic lobe neuroepithelia in larvae.

(Richter et al., 2011)

When ban^{D.Scer\UAS.T:Avic\GFP-EGFP} is driven by Scer\GAL4^en-e16E overgrowth is seen in the posterior compartment of the wing and of segments in the larval body.

(Brennecke et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, mir-ban^D.UAS.EGFP has abnormal neuroanatomy | larval stage phenotype, suppressible by Myc^KK103869, Scer\GAL4^insc-Mz1407

(Wu et al., 2017)

Enhancer of

Statement

Reference

mir-ban^D.UAS.EGFP, Scer\GAL4^insc-Mz1407 is an enhancer of abnormal neuroanatomy | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Wu et al., 2017)

mir-ban^D.UAS.EGFP, Scer\GAL4^insc-Mz1407 is an enhancer of increased cell number | larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Wu et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^vas.PU/mir-ban^D.UAS.EGFP is a non-enhancer of increased cell number | oogenesis phenotype of Scer\GAL4^vas.PU, sisRNA:2^RNAi.UAS.1

(Osman and Pek, 2018)

Suppressor of

Statement

Reference

mir-ban^D.UAS.EGFP, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/mir-ban^D.UAS.EGFP is a non-suppressor | somatic clone of increased cell number | somatic clone | larval stage phenotype of Scer\GAL4^Act.PU, numb^TS4D.UAS

(Wu et al., 2017)

Scer\GAL4^Act.PU/mir-ban^D.UAS.EGFP is a non-suppressor | somatic clone of abnormal neuroanatomy | somatic clone | larval stage phenotype of Scer\GAL4^Act.PU, numb^TS4D.UAS

(Wu et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, mir-ban^D.UAS.EGFP has intermediate neuronal progenitor | larval stage phenotype, suppressible by Myc^KK103869, Scer\GAL4^insc-Mz1407

(Wu et al., 2017)

Scer\GAL4^insc-Mz1407, mir-ban^D.UAS.EGFP has nucleolus | larval stage phenotype, suppressible by Myc^KK103869, Scer\GAL4^insc-Mz1407

(Wu et al., 2017)

Enhancer of

Statement

Reference

mir-ban^D.UAS.EGFP, Scer\GAL4^insc-Mz1407 is an enhancer of type II neuroblast | larval stage | increased number phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Wu et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^vas.PU/mir-ban^D.UAS.EGFP is a non-enhancer of female germline stem cell phenotype of Scer\GAL4^vas.PU, sisRNA:2^RNAi.UAS.1

(Osman and Pek, 2018)

Suppressor of

Statement

Reference

mir-ban^D.UAS.EGFP, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

mir-ban^D.UAS.EGFP, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Bilen et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/mir-ban^D.UAS.EGFP is a non-suppressor | somatic clone of type II neuroblast | increased number | somatic clone | larval stage phenotype of Scer\GAL4^Act.PU, numb^TS4D.UAS

(Wu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Myc^KK103869 suppresses the increased nucleolar size seen in secondary neuroblasts in larval brains expressing ban^{D.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^insc-Mz1407.

Co-expression of ban^{D.Scer\UAS.T:Avic\GFP-EGFP} leads to enhancement of the ectopic neuroblast formation and increased neuroblast number phenotypes seen in larval brains expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

Co-expression of ban^{D.Scer\UAS.T:Avic\GFP-EGFP} fails to suppress the ectopic type II neuroblast phenotype seen in larval brain clones expressing numb^{TS4D.Scer\UAS} under the control of Scer\GAL4^Act.PU.

(Wu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Expression of ban^{D.Scer\UAS.T:Avic\GFP-EGFP}, bearing only a 100bp region containing the ban miRNA suppresses degeneration in Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} Scer\GAL4^GMR.PF flies, restoring external and internal eye structures towards normal.

(Bilen et al., 2006)

Complementation and Rescue Data

Rescues

mir-ban^D.UAS.EGFP rescues Df(3L)mir-ban^Δ1

(Brennecke et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ban^{D.Scer\UAS.T:Avic\GFP-EGFP}

ban^D.UAS.EGFP

mir-ban^D.UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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