spindle & oocyte
Less than 1% of taccstella592 embryos are viable, and those that do develop exhibit a high incidence of mitotic defects, for example, extra centrosomes at the cortex during nuclear cycle 14.
taccstella592 females have disorganised meiotic spindles in the oocyte.
taccstella592 has spindle & oocyte phenotype, enhanceable by sub1
taccstella592 is an enhancer of spindle & oocyte phenotype of sub1
balltrip taccstella592 double mutants exhibit a phenotype identical to balltrip single mutants and no taccstella592 phenotype, indicating that balltrip is epistatic to taccstella592.
sub1 ; taccstella592 double mutant females have severe spindle formation defects in the oocyte, more dramatic than either single mutant. There are often multiple bundles of microtubules in the double mutants, some associating with chromosomes, although most bundles of microtubules are randomly organised.
taccstella592 is rescued by taccUbi-p63E.GFP
taccstella592 is partially rescued by taccS863L.Ubi-p63E.GFP
Expression of taccUbi-p63E.T:Avic\GFP in a taccstella592 mutant background results in approximately 90% viability, with these embryos exhibiting very few mitotic defects.
Expression of taccS863L.Ubi-p63E.T:Avic\GFP in a taccstella592 mutant background results in approximately 30% viability, with those embryos that do develop exhibiting a high incidence of mitotic defects, suggesting only partial rescue.
B. Williams M. Goldberg
