FB2024_03 , released June 25, 2024
Allele: Dmel\Mbshs.PT
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General Information
Symbol
Dmel\Mbshs.PT
Species
D. melanogaster
Name
heat shock construct of Tan
FlyBase ID
FBal0144745
Feature type
allele
Associated gene
Dmel\Mbs
Associated Insertion(s)
Carried in Construct
P{hs-Mba.T}
Also Known As
hsDMYPT
Key Links
Transgenic product class
cDNA
(Tan et al., 2003)
wild_type
(Tan et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Tan et al., 2003)
wild_type
(Tan et al., 2003)
Mutagen
in vitro construct
(Tan et al., 2003)
Progenitor genotype
Carried in construct
P{hs-Mba.T}
Cytology
Description

Expression of the Mbs ORF (from cDNA AT12677) is under the control of a heat shock promoter.

(Tan et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Mbs
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous Mbs03802 flies expressing Mbshs.PT at the start of embryogenesis (to rescue lethality) are fertile. Depletion of Mbshs.PT results in the formation of small ring canals at stage 10 but has no effect on the number of mitotic divisions or on the morphology of the fusome network. In contrast, the scra-stained nascent ring canals in the homozygous mutants are significantly smaller than the corresponding rings in the heterozygous flies at cyst stage IV-f, prior to ring canal growth.

      (Ong et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Mbshs.PT rescues Mbs03802

      (Tan et al., 2003)
      Comments

      Mbshs.PT rescues Mbs03802 homozygotes to adulthood when the animals are heat shocked for one hour each day during their development. If heat shocking ceases 2 days before eclosion the rescue is incomplete, with surviving adults developing wing and leg defects.

      (Tan et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mbshs.PT
      Name Synonyms
      heat shock construct of Tan
      (Tan et al., 2003)
      Secondary FlyBase IDs
        References (3)