A BstEII site at position 455 in the nos cDNA has been end-filled and re-ligated to create a frameshift closely followed by a translation stop codon.
Flies expressing nosΔB.αTub84B.3'UTR.nos+2.Scer\UAS under the control of Scer\GAL4sca-109-68 or Scer\GAL4Eh.2.4 do not show an "adolescent" phenotype (this phenotype is failure of the wing expansion and body cuticle hardening that normally occurs shortly after eclosion).