The premature stop codon is within the second zinc finger motif of jing.
Amino acid replacement: W1200term.
G6618087A
W1200term | jing-PD; W1200term | jing-PH; W1458term | jing-PI; W1200term | jing-PJ
W1200term
G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
In control stage 15 embryos, the anterior and posterior lateral trunks (Lta and Ltp, respectively) from adjacent segments are joined. In contrast, gaps occur between the Lta and Ltp in stage 15 homozygous jing1 embryos.
By stage 15, in control embryos, tracheal ganglionic cells and branches enter the ventral nerve cord (VNC) toward the central nervous system (CNS) midline. In contrast, three ganglionic branch (Gb) phenotypes are observed in in jing1 homozygotes: (1) Gbs do not reach the CNS midline appearing truncated; (2) Gbs show breaks; and (3) Gbs are absent from ventral positions
An additional phenotype observed in jing1 mutant embryos is the aberrant presence of Db cells in adjacent tracheal metameres instead of near the dorsal midline as in control embryos.
jing[+]/jing1, vvldfr-E82 has adult dorsal tracheal branch | embryonic stage phenotype
jing1, vvldfr-E82/vvl[+] has tracheal lateral trunk anterior branch primordium | embryonic stage 15 phenotype
jing1, vvldfr-E82/vvl[+] has embryonic/larval ganglionic tracheal branch | embryonic stage 15 phenotype
jing1, pnt[+]/pntΔ88 has tracheal lateral trunk posterior branch primordium | embryonic stage 15 phenotype
jing1, pnt[+]/pntΔ88 has embryonic/larval ganglionic tracheal branch | embryonic stage 15 phenotype
In contrast to single heterozygous vvldfr-E82 or jing1 mutants, double heterozygous embryos show tracheal ganglionic branch defects.
In contrast to single heterozygous pntΔ88 or jing1 mutants, double heterozygous embryos show tracheal ganglionic branch defects.
In contrast to each single heterozygote, abnormal Db boundary crossovers are observed in about 12% of metameres in jing1/vvldfr-E82 double heterozygotes.
Phenotypic analysis suggests the following allelic series: jing3 > jing2 > jing1.