FB2024_03 , released June 25, 2024
Allele: Dmel\lmdA388
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General Information
Symbol
Dmel\lmdA388
Species
D. melanogaster
Name
FlyBase ID
FBal0134061
Feature type
allele
Associated gene
Dmel\lmd
Associated Insertion(s)
Carried in Construct
Also Known As
mincA388
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Ruiz-Gomez et al., 2002)
Mutagen
ethyl methanesulfonate
(Ruiz-Gomez et al., 2002)
Progenitor genotype
Cytology
Description

Nucleotide substitution: G959A.

(Ruiz-Gomez et al., 2002)

Amino acid replacement: W320term.

(Ruiz-Gomez et al., 2002)

Amino acid replacement: F140I.

(Ruiz-Gomez et al., 2002)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:23,026,034..23,026,034 [-]
Nucleotide change:

T23026034A

Amino acid change:

F140I | lmd-PB

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Mutation is 1 of 2 in strain. Could be polymorphism.

(Ruiz-Gomez et al., 2002)
point_mutation
3R:23,025,493..23,025,493 [-]
Nucleotide change:

G23025493A

Reported nucleotide change:

G959A

Amino acid change:

W320term | lmd-PB

Reported amino acid change:

W320term

Comment:

Reported nucleotide change refers to the cDNA. Difference thought to be responsible for mutant phenotype. Other differences thought to be polymorphic variants.

(Ruiz-Gomez et al., 2002)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The heart develops normally in mutant embryos. Mutant embryos show a complete lack of myoblast fusion and all somatic muscles are mononucleated. Fusion also does not occur in the pharynx muscles. The somatic founder myoblasts appear to differentiate normally, elongating and attaching to the epidermis at the correct insertion sites. Fusion-competent myoblasts are not detected at any stage with Myosin staining. Syncytial visceral muscles are not formed in mutant embryos.

      (Ruiz-Gomez et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      lmdA388 has embryonic myoblast phenotype, non-suppressible by Scer\GAL4twi.PB/Mef2III.UAS

      (Ruiz-Gomez et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      lmdA388 is partially rescued by Scer\GAL4twi.PB/lmdUAS.cRa

      (Ruiz-Gomez et al., 2002)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      lmdA388
      (Popichenko et al., 2013)
      mincA388
      (Popichenko et al., 2013, Marin et al., 2004, Ruiz-Gomez et al., 2002)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)