FlyBase Allele Report: Dmel\Pka-R1[BDK.UAS.Tag:HA]

General Information

Symbol

Dmel\Pka-R1^{BDK.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0126505

Feature type

allele

Associated gene

Dmel\Pka-R1

Associated Insertion(s)

Carried in Construct

P{UAS-HA-Pka-R1.BDK}

Also Known As

UAS-PKA^inh

Key Links

Transgenic product class

missense_variant

(Kiger and O'Shea, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kiger and O'Shea, 2001)

Mutagen

in vitro construct

(Kiger and O'Shea, 2001)

Progenitor genotype

Carried in construct

P{UAS-HA-Pka-R1.BDK}

Cytology

Description

UAS regulatory sequences drive expression of Pka-R1 with mutations in the cAMP binding sites (amino acid replacements G196E and G321D) and tagged at the N-terminal end with Tag:HA.

(Kiger and O'Shea, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pka-R1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | drug conditional, with Scer\GAL4^c107

(Rodan et al., 2002)

abnormal locomotor behavior | drug conditional, with Scer\GAL4^Tab2-201Y

(Rodan et al., 2002)

abnormal sleep, with Scer\GAL4^17d

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^50y

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^104Y

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^Alp4-c507

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^c309

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^c747

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^{CenB1A-Mai301}

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^H24

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^Ilp2.PR

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^{Mef2.247.Switch}

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^MJ63

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^Mz1366

(Crocker et al., 2010)

abnormal sleep, with Scer\GAL4^Toll-6-D42

(Crocker et al., 2010)

chemical resistant, with Scer\GAL4^c107

(Rodan et al., 2002)

chemical resistant, with Scer\GAL4^Mhc.PW

(Rodan et al., 2002)

chemical resistant, with Scer\GAL4^Tab2-201Y

(Rodan et al., 2002)

chemical sensitive, with Scer\GAL4^CrzR.3.5.PS

(Sha et al., 2014)

chemical sensitive, with Scer\GAL4^hs.PB

(Rodan et al., 2002)

increased occurrence of cell division | adult stage, with Scer\GAL4^rk.pan

(Scopelliti et al., 2014)

lethal, with Scer\GAL4^JW1

(Kiger and O'Shea, 2001)

lethal, with Scer\GAL4^KO5

(Kiger and O'Shea, 2001)

lethal, with Scer\GAL4^PP3

(Kiger and O'Shea, 2001)

lethal, with Scer\GAL4^RK5

(Kiger and O'Shea, 2001)

visible, with Scer\GAL4^30A

(Kiger and O'Shea, 2001)

visible, with Scer\GAL4^RZ4

(Kiger and O'Shea, 2001)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^CCAP.PP

(Luan et al., 2006)

adult midgut, with Scer\GAL4^rk.pan

(Scopelliti et al., 2014)

intestinal stem cell | adult stage, with Scer\GAL4^rk.pan

(Scopelliti et al., 2014)

wing, with Scer\GAL4^CCAP.PP

(Luan et al., 2006)

wing, with Scer\GAL4^RZ4

(Kiger and O'Shea, 2001)

wing | ectopic, with Scer\GAL4^30A

(Kiger and O'Shea, 2001)

wing disc anterior compartment, with Scer\GAL4^ap-md544

(Kiger and O'Shea, 2001)

Detailed Description

Statement

Reference

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^rk.pan results in intestinal stem cell hyperproliferation in the adult midgut.

(Scopelliti et al., 2014)

Expression of Pka-R1^{BDK.UAS.Tag:HA} in Crz neurons under the control of Scer\GAL4^CrzR.3.5.PS results in flies that show a significantly delayed recovery from ethanol-induced sedation.

(Sha et al., 2014)

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{Mef2.247.Switch}, Scer\GAL4^H24, Scer\GAL4^c747, Scer\GAL4^17d, Scer\GAL4^c309, Scer\GAL4^Mz1366, Scer\GAL4^MJ63, Scer\GAL4^Aph-4-c507, Scer\GAL4^104Y, Scer\GAL4^D42, Scer\GAL4^{cenB1A-Mai301}, or Scer\GAL4^50y increases the amount of total night-time sleep, due to Pka-R1 expression blocking PKA signalling.

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} in a subset of PI neurons under the control of Scer\GAL4^Ilp2.PR results in a significant increase in sleep consolidation - i.e. a decreased number of sleep bouts with a corresponding increase in sleep bout length. However, total nighttime sleep is not increased.

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^30Y, Scer\GAL4^Sep54, Scer\GAL4^Tab2-201Y, Scer\GAL4^Ilp2.PR, Scer\GAL4^{elav.Switch.PO} or Scer\GAL4^Kurs58 has no effect on PKA signalling and the nighttime sleep modulation in response to octopamine.

(Crocker et al., 2010)

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Ccap.PP completely inhibits both wing expansion and tanning. Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^dimm-929 does not have a significant effect on wing expansion or cuticle tanning.

(Luan et al., 2006)

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^Tab2-201Y, Scer\GAL4^c107 or Scer\GAL4^c522 leads to a decrease in ethanol sensitivity. The levels of absorbed ethanol is not affected in these flies. Decreased ethanol sensitivity of Scer\GAL4^Tab2-201Y>Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} flies remains after mushroom body ablation. Flies expressing Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Tab2-201Y, and to a lesser extent when under the control of Scer\GAL4^c107, are resistant to the locomotor-depressant effects of ethanol. In contrast, when expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} is driven by Scer\GAL4^c522 or Scer\GAL4^c290, ethanol-induced locomotor effects are not significantly different from wild type. Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of both Scer\GAL4^Tab2-201Y and Scer\GAL4^c747 supresses the ethanol resistance seen when only Scer\GAL4^Tab2-201Y is used as a driver. Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} driven by the following lines does not affect sensitivity of flies to ethanol: Scer\GAL4^c309, Scer\GAL4^c747, Scer\GAL4^c739, Scer\GAL4^c758, Scer\GAL4^MJ162A, Scer\GAL4^C-765, Scer\GAL4^c346, Scer\GAL4^064Y, Scer\GAL4^c767, Scer\GAL4^C41, Scer\GAL4^c119, Scer\GAL4^c491, Scer\GAL4^c453, Scer\GAL4^104Y, Scer\GAL4^OK348, Scer\GAL4^c687, Scer\GAL4^007Y, Scer\GAL4^078Y, Scer\GAL4^Mz423, Scer\GAL4^c229, Scer\GAL4^Okt30, Scer\GAL4^Feb204, Scer\GAL4^Kurs58, Scer\GAL4^Jan129, Scer\GAL4^Mai301, Scer\GAL4^Jan229, Scer\GAL4^Kurs21, Scer\GAL4^Mai179, Scer\GAL4^Kurs6, Scer\GAL4^OK086, Scer\GAL4^OK028, Scer\GAL4^OK062, Scer\GAL4^OK144D, Scer\GAL4^OK144L, Scer\GAL4^OK165, Scer\GAL4^OK168, Scer\GAL4^OK175, Scer\GAL4^OK309, Scer\GAL4^elav.PLu, Scer\GAL4^c123a, Scer\GAL4^Mhc.PW, Scer\GAL4^c290, Scer\GAL4^121Y, Scer\GAL4^17d, Scer\GAL4^c302, Scer\GAL4^c584, Scer\GAL4^c819, Scer\GAL4^c81, Scer\GAL4^c105, Scer\GAL4^c161, Scer\GAL4^c217, Scer\GAL4^c338, Scer\GAL4^c401a, Scer\GAL4^c481, Scer\GAL4^c628, Scer\GAL4^Feb170, Scer\GAL4^c159b, Scer\GAL4^c259, Scer\GAL4^c061, Scer\GAL4^Aph-4-c232, Scer\GAL4^c561, and Scer\GAL4^c548. Heat shock-induced expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1}, driven by Scer\GAL4^hs.PB, results in increased sensitivity to ethanol.

(Rodan et al., 2002)

Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ap-md544 results in outgrowth of the anterior compartment of the wing disc. Expression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^30A results in the formation of a small anterior mirror-image wing duplication in the costal region.

(Kiger and O'Shea, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pka-R1^{BDK.UAS.Tag:HA}, Scer\GAL4^Tab2-201Y has chemical resistant phenotype, suppressible by Pka-C1^UAS.Tag:FLAG, Scer\GAL4^Tab2-201Y

(Rodan et al., 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} and Pka-C1^{Scer\UAS.T:Zzzz\FLAG}, both under the control of Scer\GAL4^Tab2-201Y, suppresses the reduced ethanol sensitivity phenotype of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1}.

(Rodan et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

D. Kalderon

(Kiger and O'Shea, 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1}

Pka-R1^{BDK.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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