synapse, with Scer\GAL4C57
Embryos derived from females expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 show a moderate but significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type.
Expression of Scer\UBP2Scer\UAS.cDa in ddaC neurons under the control of Scer\GAL4ppk.1.9 results in dendrites that remain for many days after severing. Approximately 83% of animals still exhibit distal dendrites 18 hours after severing.
Pruning of ddaC dendrites is delayed upon expression of Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4ppk.1.9.
Overexpression of Scer\UBP2Scer\UAS.cDa in presynaptic neurons by Scer\GAL4OK6 does not affect synapse elimination.
Overexpression of Scer\UBP2Scer\UAS.cDa in postsynaptic muscles by Scer\GAL4C57 results in normal synaptic growth but delayed synaptic elimination.
Overexpression of Scer\UBP2Scer\UAS.cDa in the C4da neurons, under the control of Scer\GAL4ppk.PG results in some of the C4da neurons aberrantly retaining their larval dendritic arbors, through the prevention of efficient severing of dendrites from the soma.
Expression of Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4elav.PLu doubles the number of boutons per muscle surface area at the neuromuscular junction compared to wild type.
Mushroom body γ neurons expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4Tab2-201Y show major ultrastructural differences to wild-type neurons during developmental axon pruning; there is a decrease in the number of vacuoles and a general decrease in the interruption of axons compared to wild type at 12 hours after puparium formation (APF). The mutant axons maintain a readily identifiable microtubule cytoskeleton even at 12 hours APF (the microtubule cytoskeleton is absent or disrupted in wild-type animals at 6-12 hours APF). In addition, in the mutant neurons, many long axon profiles with wider diameters persist at 12 hours APF (in wild-type animals the length and diameter of axons appear to decrease during pruning). The frequency of multilamellar bodies is greatly reduced in the mutant neurons. The invasion of glical cells into the mushroom body lobes that is seen during developmental axon pruning in early pupae is unaffected in animals expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4Tab2-201Y.
When Scer\UBP2Scer\UAS.cDa is driven by Scer\GAL4Tab2-201Y in the Mushroom Body γ neurons, during late larval stages, mutant neurons exhibit largely normal growth and guidance of axons. However at 18hr APF, the peak of axon pruning, mutant neurons fail to prune their axons. As a consequence γ neurons retain their dorsal lobes in the adult. When Scer\UBP2Scer\UAS.cDa is driven in the brain using either Scer\GAL4Tab2-201Y or Scer\GAL4OK107 as a driver, no effect is seen on cell proliferation.
Over expression of Scer\UBP2Scer\UAS.cDa in the nervous system (driven by Scer\GAL4elav-C155) leads to a marked synaptic overgrowth and a severe reduction in presynaptic transmitter release.
Scer\GAL4ey-OK107/Scer\UBP2UAS.cDa is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of bskflp147E
Scer\GAL4ey-OK107/Scer\UBP2UAS.cDa is a non-suppressor of alpha/beta Kenyon cell phenotype of bskflp147E
Scer\GAL4ey-OK107/Scer\UBP2UAS.cDa is a non-suppressor of gamma Kenyon cell phenotype of bskflp147E
Expression of Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4ey-OK107 fails to suppress the axon degeneration seen in bskflp147E mutant mushroom body neuron clones.
The increase in bouton number at the neuromuscular junction seen in larvae expressing Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4elav.PLu in a wild-type background is not seen if they express Scer\UBP2Scer\UAS.cDa under the control of Scer\GAL4elav.PLu in a witunspecified or Medunspecified background.