P{GT1}roboBG01092 is located in the first intron of robo within the 5' untranslated region.
Insertion within the robo transcription unit.
Insertion is in the robo gene.
commissure (with robo11)
The adult sLNv neurons of robo11/robo1BG01092 transheterozygotes do not show significant axon length differences compared to controls.
roboBG01092 homozygous flies show a consistent and significant shortening of the period of the behavioural rhythm compared to wild-type controls under free-running conditions.
Heterozygous roboBG01092 mutants exhibit largely normal behavioural rhythms.
roboBG01092 mutant flies exhibit a similar avoidance response to benzaldehyde as in wild-type.
roboBG01092 mutants display normal eclosion behaviour.
Homozygous roboBG01092 mutants exhibit axon guidance defects. The overall pattern of the CNS axon scaffold is fairly normal; the longitudinal axon tracts as well as the anterior and posterior commissures are spaced normally and usually have normal thickness. The main defect observed is related to the anterior defasciculation of the longitudinal tracts, whereby in certain segments up to five axonal bundles can be observed. in addition, commissural bundles in some segments appear slightly abnormal and wander between commissures, extending their projections along and over the midline instead of projecting towards the contralateral side, resulting in a distinct phenotype. Quantification reveals that the most prominent defect in roboBG01092 individuals is the defasciculation of longitudinal axon bundles.
roboBG01092 responds to a light pulse during the early night (ZT 15) with an approximate 3hr delay, as in wild-type, although the phase shift in mutant flies is larger than in wild-type. After ZT 21, the magnitude of the hase respose is slightly smaller in roboBG01092 individuals compared to wild-type.
After 10 days under synchronizing temperature cycles (25-20[o]C, where the warmer temperatures are reminiscent of the light period), where roboBG01092 flies disply close to 24hr rhythms under temperature entrainment, as wild-type, the endogenous free-running period is significantly shorter in roboBG01092 homozygotes than in wild-type controls
roboBG01092 mutants display a similar morning anticipation as wild-type in a 12hr LD regime. However the anticipation of the evening transition starts at least half an hour earlier than in wild-type controls, consistent with the shorter periodicity in the activity rhythms in free-running conditions.
Small LNv neurons are mislocalised in roughly half of the roboBG01092 brain hemispheres analysed. Misplaced neurons are located in the dorsal protocerebrum and occasionally found next to the large LNvs. Supernumerary cells are not detected in pdf-positive clusters roboBG01092 brains. Subtly altered axon trajectories are found in a high proportion of the dissected roboBG01092 brains. The axon bundle running along the posterio optic tract does not run in the same way in roboBG01092 brains as in wild-type. To quantify this difference the angle between the initial turn of the axon fibre and a base line with the same origin that reaches to an intermediate point in the posterior optic tract just on top of the esophageal foramen at the midline. Using this assay, wild-type brains tend to display 'negative' angles, while most roboBG01092 brains exhibit 'positive' angles.
roboBG01092 brains show no apparent difference in the trajectory of photoreceptor axons at the level of the optic lobe.
roboBG01092 is homozygous viable.
roboBG01092/robo1 transheterozygotes exhibit ectopic midline crossing.
Homozygous males and females show a significant increase in starvation resistance compared to controls.
Mutants exhibit a quantitative gain of bristles.
robo1BG01092 is an enhancer of abnormal circadian rhythm phenotype of Pdf01
roboBG01092 Pdf01 double mutants exhibit a lengthening of the free-running period in a dose-dependent manner. Introducing a copy of roboBG01092 into Pdf01 mutants gives rise to >80% rhythmic individuals. The average activity plot of roboBG01092 Pdf01 flies shows a modest increase in the activity ~1hr before the lights-on transition, indicating that the morning anticipation is rescued even in the absence of Pdf neuropeptide.
roboBG01092 Pdf01 double mutants show a significantly lengthened period of locomotor activity.
Transheterozygous roboBG01092 Pdf01 flies display wild-type period and rhythmicity.
robo1BG01092 is rescued by robo1UAS.Tag:MYC/Scer\GAL4tim.PE
