FB2024_03 , released June 25, 2024
Allele: Dmel\dshEGFP
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General Information
Symbol
Dmel\dshEGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0123908
Feature type
allele
Associated gene
Dmel\dsh
Associated Insertion(s)
Carried in Construct
P{dsh.EGFP}
Also Known As
Dsh-GFP, Dsh::GFP, Dsh:GFP, DshGFP
Key Links
Transgenic product class
genomic_DNA
(Axelrod, 2001)
wild_type
(Axelrod, 2001)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Axelrod, 2001)
wild_type
(Axelrod, 2001)
Mutagen
in vitro construct
(Axelrod, 2001)
Progenitor genotype
Carried in construct
P{dsh.EGFP}
Cytology
Description

6.7kb genomic fragment containing dsh tagged at the 3' end with EGFP.

(Axelrod, 2001)
Allele components
Component
Use(s)
Regulatory region(s)
dsh
dsh_Dsh::GFP
Encoded product / tool
dsh
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      pupal stage P7
      cell | distal of arista

      Comment: reference states 36 hr APF

      (He and Adler, 2002)
      Additional Information
      Statement
      Reference

      dshT:AvicGFP-EGFP protein is localized preferentially to the distal side of cells comprising the arista during pupal development.

      (He and Adler, 2002)
       
      Marker for
      Reflects expression of
      dsh
      (He and Adler, 2002)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing dshT:Avic\GFP-EGFP in a dsh3 background exhibit wild type PCP patterns in all tissues examined.

      (Yanfeng et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      dshEGFP is a suppressor | partially of wing margin phenotype of Scer\GAL4en-e16E, fz1-1-1.UAS.Tag:MYC

      (Wu et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      dshEGFP rescues dsh1

      (Capilla et al., 2012)

      dshEGFP rescues dsh3

      (Yanfeng et al., 2011)

      dshEGFP rescues dsh3

      (Axelrod, 2001)
      Partially rescues

      dshEGFP partially rescues dsh3

      (Capilla et al., 2012)
      Comments

      dshT:Avic\GFP-EGFP rescues the ectopic leg joint phenotype of dsh1 animals.

      Some dsh3 animals rescued to adulthood by dshT:Avic\GFP-EGFP show an ectopic leg joint phenotype.

      (Capilla et al., 2012)

      Expression of dshT:Avic\GFP-EGFP fully rescues the lethality and PCP defects seen in dsh3 mutants.

      (Yanfeng et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      dshEGFP
      dshT:Avic\GFP-EGFP
      dshT:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (22)