FlyBase Allele Report: Dmel\grim[C.UAS]

General Information

Symbol

Dmel\grim^C.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0123849

Feature type

allele

Associated gene

Dmel\grim

Associated Insertion(s)

Carried in Construct

P{UAS-grimC}

Also Known As

UAS-grimC

Key Links

Transgenic product class

inframe_deletion

(Wing et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Wing et al., 2001)

Mutagen

in vitro construct

(Wing et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-grimC}

Cytology

Description

Amino acids 15 to 138 of grim are driven by UASt sequences.

(Wing et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

grim

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Wing et al., 2001)

peptidergic neuron | third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2013)

PNS glial cell | embryonic stage, with Scer\GAL4^elav-C155

(Sepp and Auld, 2003)

PNS glial cell | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

sensory neuron & axon & embryo, with Scer\GAL4^elav-C155

(Sepp and Auld, 2003)

sensory neuron & axon & embryo, with Scer\GAL4^repo

(Sepp and Auld, 2003)

Detailed Description

Statement

Reference

Expression of grim^C.Scer\UAS under the control of Scer\GAL4^CCAP.PP induces partial death of CCAP neurons in the third instar larval CNS.

(Lee et al., 2013)

Expression of grim^C.Scer\UAS, under control of the post-mitotic neuron-specific Scer\GAL4^elav-C155 driver in embryos, causes apoptosis of sensory neurons and a corresponding loss of peripheral glial cells. Many of these neurons stray off course and have misrouted axons. In embryos where axons do not connect between the dorsolateral region of the embryo to the CNS, the peripheral glia fail to migrate out of the CNS-PNS transition zone. Expression of grim^C.Scer\UAS under the control of the glial-specific Scer\GAL4^repo driver results in the loss of sensory neurons; remaining neurons often have shortened or misrouted axons, or show complete failure of axogenesis.

(Sepp and Auld, 2003)

grim^C.Scer\UAS when driven by Scer\GAL4^Gp150-52A does not induce midline cell death, but when driven by Scer\GAL4^GMR.PF, ectopic eye cell death does occur.

(Wing et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, grim^C.UAS has increased cell death phenotype, suppressible | partially by Dronc^C318S.GMR

(Wing et al., 2001)

Scer\GAL4^GMR.PF, grim^C.UAS has visible phenotype, suppressible | partially by Dronc^C318S.GMR