FB2024_03 , released June 25, 2024
Allele: Dmel\Uba2C175S.UAS
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General Information
Symbol
Dmel\Uba2C175S.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0121879
Feature type
allele
Associated gene
Dmel\Uba2
Associated Insertion(s)
Carried in Construct
P{UAS-Uba2.C175S}
Key Links
Transgenic product class
missense_variant
(Long and Griffith, 2000)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Long and Griffith, 2000)
Mutagen
in vitro construct
(Long and Griffith, 2000)
Progenitor genotype
Carried in construct
P{UAS-Uba2.C175S}
Cytology
Description

UAS regulatory sequences drive expression of a mutated form of Uba2.

(Long and Griffith, 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Uba2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Kennedy's disease
      modeled by Hsap\ARQ112.UAS.Tag:HA
      (Chan et al., 2002)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Uba2C175S.Scer\UAS driven by Scer\GAL4GMR.PF results in a variable slightly glossy, rough eye phenotype, sometimes with loss of pigmentation. This is temperature-dependent.

      (Chan et al., 2002)

      Expression of one copy of Uba2C175S.Scer\UAS under the control of Scer\GAL4elav-C155 generally results in the production of apparently normal adult progeny with no or very mild lethality at 29oC. P{UAS-Uba2.C175S} lines expressing higher levels of Uba2C175S.Scer\UAS result in higher lethality (approximately 50% for P{UAS-Uba2.C175S}117F and 100% for P{UAS-Uba2.C175S}92E). Animals expressing high levels of Uba2C175S.Scer\UAS under the control of Scer\GAL4GMR.PF have a rough, depigmented eye.

      (Long and Griffith, 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4GMR.PF, Uba2C175S.UAS has increased cell death | temperature conditional phenotype, enhanceable by Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF

      (Chan et al., 2002)
      NOT suppressed by
      Statement
      Reference

      Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF, Uba2C175S.UAS has increased cell death | temperature conditional phenotype, non-suppressible by Hsap\HSPA1LUAS.cWa, Scer\GAL4GMR.PF

      (Chan et al., 2002)
      Enhancer of
      NOT Enhancer of
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4GMR.PF, Uba2C175S.UAS has eye | temperature conditional phenotype, enhanceable by Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF

      (Chan et al., 2002)

      Scer\GAL4GMR.PF, Uba2C175S.UAS has ommatidium | temperature conditional phenotype, enhanceable by Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF

      (Chan et al., 2002)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The eye phenotype resulting from simultaneous expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 and Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4GMR.PU does not appear to be affected by co-expression of Uba2C175S.Scer\UAS.

      The Hsp60DdsRNA.Sym.Scer\UAS-mediated suppression of the eye phenotype resulting from the expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4GMR.PU is less pronounced in the presence of Uba2C175S.Scer\UAS.

      Simultaneous expression of Uba2C175S.Scer\UAS and Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU enhances eye degradation, resulting in more glazed eyes with little pigmentation.

      Simultaneous expression of Uba2C175S.Scer\UAS and Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU enhances the eye degeneration phenotype.

      (Arya et al., 2010)

      Co-expression of Uba2C175S.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (both under the control of Scer\GAL4GMR.PF) results in a constant enhancement of the eye degeneration, as illustrated by severe loss of pigmentation.

      Co-expression of Uba2C175S.Scer\UAS and Hsap\HSPA1LScer\UAS.cWa with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (all under the control of Scer\GAL4GMR.PF) fails to suppress the enhancement of the eye degeneration phenotype found in Uba2C175S.Scer\UAS ; Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 flies.

      (Chan et al., 2002)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Uba2C175S.Scer\UAS
      Uba2C175S.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)