FlyBase Allele Report: Dmel\per[UAS.cYa]

General Information

Symbol

Dmel\per^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Yang

FlyBase ID

FBal0121609

Feature type

allele

Associated gene

Dmel\per

Associated Insertion(s)

Carried in Construct

P{UAS-per.Y}

Also Known As

UAS-per

Key Links

Transgenic product class

cDNA

(Yang and Sehgal, 2001)

wild_type

(Yang and Sehgal, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Yang and Sehgal, 2001)

wild_type

(Yang and Sehgal, 2001)

Mutagen

in vitro construct

(Yang and Sehgal, 2001)

Progenitor genotype

Carried in construct

P{UAS-per.Y}

Cytology

Description

Expression of full length per cDNA is governed by UAS regulatory sequences.

(Yang and Sehgal, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

per

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^P2.4.Pdf

(Beckwith et al., 2013)

abnormal circadian rhythm | adult stage, with Scer\GAL4^Pdf.PU

(Xue et al., 2019)

abnormal circadian rhythm | adult stage, with Scer\GAL4^tim.PU

(Xue et al., 2019)

abnormal locomotor rhythm, with Scer\GAL4^Act5C.PI

(Yang and Sehgal, 2001)

abnormal locomotor rhythm, with Scer\GAL4^cry.PZ

(Zhao et al., 2003)

abnormal locomotor rhythm, with Scer\GAL4^elav-C155

(Yang and Sehgal, 2001)

abnormal locomotor rhythm, with Scer\GAL4^elav-C155, Scer\GAL80^cry.PS, per⁰¹

(Stoleru et al., 2004)

abnormal locomotor rhythm, with Scer\GAL4^P0.5.Pdf

(Collins et al., 2006)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Yang and Sehgal, 2001)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS

(Stoleru et al., 2007)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^P2.4.Pdf

(Beckwith et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of per^UAS.cYa under the control of Scer\GAL4^tim.PU induces a decrease in circadian rhythmicity. Expression under the control of Scer\GAL4^Pdf.PU induces a shortened circadian period.

(Xue et al., 2019)

Flies expressing per^Scer\UAS.cYa under the control of Scer\GAL4^P2.4.Pdf show similar locomotor activity period under free-running conditions (total darkness) as controls but the percentage as well as the rhythmic power of the behavioral oscillations is somewhat decreased.

(Beckwith et al., 2017)

Overexpression of per^Scer\UAS.cYa under the control of Scer\GAL4^P2.4.Pdf leads to a long period phenotype.

(Beckwith et al., 2013)

Flies expressing per^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE in a Scer\GAL80^Pdf.PS background show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions.

(Stoleru et al., 2007)

Flies expressing per^Scer\UAS.cYa, under the control of Scer\GAL4^P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 22.2% versus 13%) although the period length is increased by approximately 1 hour.

(Collins et al., 2006)

Flies mutant for per⁰¹ expressing per^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 and repressed by Scer\GAL80^Pdf.PS in Pdf expressing cells, become gradually arrhythmic under DD conditions. These flies however, manifest a wild-type-like anticipation of lights-on as well as lights off peak in LD.

(Stoleru et al., 2004)

When per^Scer\UAS.cYa is driven by Scer\GAL4^cry.PP no induction of ectopic clocks is seen. A reduction is seen behavioural rhythmicity in DD conditions.

(Zhao et al., 2003)

When expression is driven in a per⁺ background by Scer\GAL4^elav-C155 or Scer\GAL4^Act5C.PI, arythmicity occurs. Two copies of P{UAS-per.Y} give a stronger effect than one. The effect is weaker with Scer\GAL4^tim.PE and not evident with Scer\GAL4^P2.4.Pdf.

(Yang and Sehgal, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^P0.5.Pdf, per^UAS.cYa has abnormal locomotor rhythm phenotype, non-enhanceable by Scer\GAL4^P0.5.Pdf/cry^UAS.cEa

(Collins et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^P2.4.Pdf, per^UAS.cYa has abnormal circadian rhythm phenotype, suppressible by shn^UAS.cMa/Scer\GAL4^P2.4.Pdf

(Beckwith et al., 2013)

Scer\GAL4^P2.4.Pdf, per^UAS.cYa has abnormal circadian rhythm phenotype, suppressible by tim^UAS.cYa/Scer\GAL4^P2.4.Pdf

(Beckwith et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^tim.PU/per^UAS.cYa is an enhancer of abnormal circadian rhythm | adult stage phenotype of Scer\GAL4^tim.PU, Sur-8^JF03236

(Xue et al., 2019)

NOT Enhancer of

Statement

Reference

Scer\GAL4^P0.5.Pdf/per^UAS.cYa is a non-enhancer of abnormal locomotor rhythm phenotype of Scer\GAL4^P0.5.Pdf, cry^UAS.cEa

(Collins et al., 2006)

Suppressor of

Statement

Reference

per^UAS.cYa, Scer\GAL4^Pdf.PU is a suppressor | partially of abnormal circadian rhythm | adult stage phenotype of Pp1-87B^HMS00409, Scer\GAL4^Pdf.PU

(Xue et al., 2019)

Scer\GAL4^tim.PU/per^UAS.cYa is a suppressor | partially of abnormal circadian rhythm | adult stage phenotype of Scer\GAL4^tim.PU, Sur-8^JF03236

(Xue et al., 2019)

per^UAS.cYa/Scer\GAL4^P2.4.Pdf is a suppressor | partially of abnormal locomotor rhythm | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Srrm1^KK108396

(Beckwith et al., 2017)

per^UAS.cYa/Scer\GAL4^P2.4.Pdf is a suppressor of abnormal circadian rhythm phenotype of Scer\GAL4^P2.4.Pdf, shn^UAS.cMa

(Beckwith et al., 2013)

per^UAS.cYa, Scer\GAL4^tim.PE is a suppressor of abnormal circadian behavior phenotype of Atx2^KK100423, Scer\GAL4^tim.PE

(Zhang et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of per^UAS.cYa suppresses the long circadian period, while enhancing the arrhythmicity, of flies expressing Sur-8^JF03236 under the combined control of Scer\GAL4^tim.PU and Dicer-2 (for more efficient RNAi).

(Xue et al., 2019)

The loss of rhythmicity in locomotor activity under free-running (constant darkness) conditions characteristic for flies expressing SRm160^KK108396 (and UAS-dicer2 to enhance the RNAi efficiency) under the control of Scer\GAL4^P2.4.Pdf is significantly improved by co-expression of per^Scer\UAS.cYa.

(Beckwith et al., 2017)

Co-expression of per^Scer\UAS.cYa and shn^Scer\UAS.cMa under the control of Scer\GAL4^P2.4.Pdf results in highly rhythmic flies, with periods close to 24 hours.

Co-expression of per^Scer\UAS.cYa and tim^Scer\UAS.cYa under the control of Scer\GAL4^P2.4.Pdf results in highly rhythmic flies, with periods close to 24 hours.

(Beckwith et al., 2013)

Expression of per^Scer\UAS.cYa suppresses the lengthening of the circadian locomotor darkness period seen when Atx2^KK100423 is expressed under the control of Scer\GAL4^tim.PE.

(Zhang et al., 2013)

Expression of cyc^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^elav-C155 in a per⁰¹, per^Scer\UAS.cYa background only shortens the circadian locomotor activity period by 20 minutes, in contrast to the shortening of 2 hours seen when cyc^{Scer\UAS.T:Hsim\VP16} is expressed under the control of Scer\GAL4^elav-C155 in a wild-type background.

(Kadener et al., 2008)

Flies expressing per^Scer\UAS.cYa and cry^Scer\UAS.cEa, under the control of Scer\GAL4^P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 15.7% versus 13%).

(Collins et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/per^UAS.cYa rescues per⁰¹

(Kaneko et al., 2012)

Scer\GAL4^tim.PE/per^UAS.cYa rescues per⁰¹

(Kaneko et al., 2012)

Scer\GAL4^elav-C155/per^UAS.cYa rescues per⁰¹

(Stoleru et al., 2004)

Partially rescues

per^UAS.cYa/Scer\GAL4^Clk.int1-3 partially rescues per⁰¹

(Kaneko et al., 2012)

per^UAS.cYa, Scer\GAL4^Clk.int1-3, Scer\GAL80^Pdf.PS partially rescues per⁰¹

(Kaneko et al., 2012)

Scer\GAL4^elav-C155/per^UAS.cYa partially rescues per⁰¹

(Kadener et al., 2008)

Scer\GAL4^elav-C155/per^UAS.cYa partially rescues per⁰¹

(Yang and Sehgal, 2001)

Fails to rescue

Scer\GAL4^elav-C155, per^UAS.cYa, Scer\GAL80^cry.PS fails to rescue per⁰¹

(Stoleru et al., 2004)

Scer\GAL4^elav-C155, per^UAS.cYa, Scer\GAL80^Pdf.PS fails to rescue per⁰¹

(Stoleru et al., 2004)

Comments

Expression of per^Scer\UAS.cYa in neuronal cells (under the control of Scer\GAL4^elav-C155) or in circadian cells (under the control of Scer\GAL4^tim.PE) rescues daytime temperature preference rhythms in per⁰¹ mutant flies.

Expression of per^Scer\UAS.cYa in M cells, under the control of Scer\GAL4^P2.4.Pdf, fails to rescue daytime temperature preference rhythm under light-dark conditions, although a weaker-amplitude rhythm with an abnormal phase is present in these flies.

Expression of per^Scer\UAS.cYa in both M and E cells with Scer\GAL4^cry.PE is unable to rescue daytime temperature preference rhythms under light-dark conditions.

Expression of per^Scer\UAS.cYa in the DN2 neurons (under the control of Scer\GAL4^Clk.int1-3) rescues approximately 46% of the per⁰¹ mutant phenotype for daytime temperature preference rhythm.

Expression of per^Scer\UAS.cYa in the DN2 neurons (under the control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS) rescues approximately 66% of the per⁰¹ mutant phenotype for daytime temperature preference rhythm.

Expression of per^Scer\UAS.cYa in DN2 neurons under the control of Scer\GAL4^Clk.int1-3 and Scer\GAL80^Pdf.PS does not rescue the per⁰¹ locomotor activity phenotypes under either light-dark or temperature cycles. These flies are also arrhythmic in dark-dark conditions.

(Kaneko et al., 2012)

Expression of per^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 can rescue the arrhythmic locomotor activity phenotype of per⁰¹ flies, with 65.2% of the rescued flies being rhythmic for locomotor activity.

(Kadener et al., 2008)

The degree of rescue of the per⁰¹ locomotor rhythm defects depends to some extent on the site of insertion of the rescuing P{UAS-per.Y} transgene and is maximally >90% and minimally >50%.

(Yang and Sehgal, 2001)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

80685

w^*; P{UAS-per.Y}3-1

80684

per⁰¹ w^*; P{UAS-per.Y}2-3

Notes on Origin

Discoverer