FlyBase Allele Report: Dmel\robo1[Y-F.UAS]

General Information

Symbol

Dmel\robo1^Y-F.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0120360

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

Carried in Construct

P{UAS-robo1.Y-F}

Also Known As

UAS-roboY-F

Key Links

Transgenic product class

missense_variant

(Bashaw et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Bashaw et al., 2000)

Mutagen

in vitro construct

(Bashaw et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-robo1.Y-F}

Cytology

Description

UAS regulatory sequences drive expression of robo1 containing a Tyr to Phe amino acid replacement in the CC1 domain.

(Bashaw et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

robo1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Kraut and Zinn, 2004)

abnormal neuroanatomy | first instar larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Ou et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

At 25-31 hours after egg laying RP2 neuron clones expressing robo1^Y-F.Scer\UAS under the control of Scer\GAL4^Act5C.PP have a lack of dendritic innervation of the medial neuropile (normally located anterior to the axon) and a concomitant expansion of dendrites in the lateral neuropile posterior to the axon.

(Ou et al., 2008)

At 29^oC, 6.1% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in larvae expressing robo^Y-F.Scer\UAS under the control of Scer\GAL4^elav-C155.

(Kraut and Zinn, 2004)

Embryos overexpressing robo^Y-F.Scer\UAS under the control of Scer\GAL4^ftz.ng show normal Fas2-axon projections.

(Hsouna et al., 2003)

Expression of robo^Y-F.Scer\UAS under the control of Scer\GAL4^elav.PLu results in a commissureless phenotype, with no axons crossing the midline.

(Bashaw et al., 2001)

Expression of robo^Y-F.Scer\UAS driven by Scer\GAL4^unspecified produces a loss of commissure phenotype.

(Bashaw et al., 2000)

Axons do not cross the midline in embryos expressing robo^Y-F.Scer\UAS under the control of Scer\GAL4^elav.PLu. Three distinct Fas2-expressing longitudinal pathways on each side of the midline are still seen. Expression of robo^Y-F.Scer\UAS under the control of Scer\GAL4^ap-md544 leads to no alteration in the lateral position of the ap-expressing neurons.

(Simpson et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, robo1^Y-F.UAS has abnormal neuroanatomy phenotype, suppressible | partially by RhoGEF64C^EP3035, Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

Suppressor of

Statement

Reference

robo1^Y-F.UAS/Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of Abl²

(Hsouna et al., 2003)

robo1^Y-F.UAS/Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of Abl⁴

(Hsouna et al., 2003)

robo1^Y-F.UAS, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Gαq^Q203L.UAS, Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, robo1^Y-F.UAS has symmetrical commissure phenotype, suppressible | partially by RhoGEF64C^EP3035, Scer\GAL4^elav.PLu

(Bashaw et al., 2001)

Scer\GAL4^unspecified, robo1^Y-F.UAS has symmetrical commissure phenotype, suppressible by ena[+]/ena^GC1

(Bashaw et al., 2000)

Suppressor of

Statement

Reference

robo1^Y-F.UAS/Scer\GAL4^ftz.ng is a suppressor of larval longitudinal connective | embryonic stage 16 phenotype of Abl²

(Hsouna et al., 2003)

robo1^Y-F.UAS/Scer\GAL4^ftz.ng is a suppressor of larval longitudinal connective | embryonic stage 16 phenotype of Abl⁴

(Hsouna et al., 2003)

robo1^Y-F.UAS, Scer\GAL4^elav-C155 is a suppressor of symmetrical commissure | embryonic stage phenotype of Gαq^Q203L.UAS, Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

robo1^Y-F.UAS, Scer\GAL4^elav-C155 is a suppressor of fascicle | embryonic stage phenotype of Gαq^Q203L.UAS, Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of robo^Y-F.Scer\UAS under the control of Scer\GAL4^ftz.ng in Abl⁴ homozygous mutants suppresses the frequency of abnormal crossovers observed in Abl⁴ mutants.

Overexpression of robo^Y-F.Scer\UAS under the control of Scer\GAL4^ftz.ng in Abl² homozygous mutants suppresses the frequency of abnormal crossovers observed in Abl² mutants.

(Hsouna et al., 2003)

In embryos expressing both Gα49B^{Q203L.Scer\UAS} and robo^Y-F.Scer\UAS under the regulation of Scer\GAL4^elav-C155, ectopic crossovers of the midline by axons.

(Ratnaparkhi et al., 2002)

Many commissural axons cross the midline, with some segments appearing nearly wild type, in embryos coexpressing robo^Y-F.Scer\UAS and Gef64C^EP3035 under the control of Scer\GAL4^elav.PLu.

(Bashaw et al., 2001)

The addition of ena^GC1/+ significantly suppresses the loss of commissure phenotype seen in robo^Y-F.Scer\UAS, Scer\GAL4^unspecified flies.

(Bashaw et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

robo1^Y-F.Scer\UAS

robo1^Y-F.UAS

robo^Y-F.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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