FlyBase Allele Report: Dmel\futsch[EP1419]

General Information

Symbol

Dmel\futsch^EP1419

Species

D. melanogaster

Name

FlyBase ID

FBal0118233

Feature type

allele

Associated gene

Dmel\futsch

Associated Insertion(s)

P{EP}futsch^EP1419

Carried in Construct

Also Known As

EP(X)1419, EP1419, UAS-futsch

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Benos et al., 2001, Gene Disruption Project members, 2001-, Zhang et al., 2001, Hummel et al., 2000, Roos et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}futsch^EP1419

Cytology

Description

The P{EP} insertion is upstream of the futsch coding region.

(Hummel et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:1,399,899..1,399,899 [+]

(Saunders et al., 2022, De Graeve et al., 2019, Dinges et al., 2017, Coyne et al., 2014, Lee et al., 2010, Gogel et al., 2006, Benos et al., 2001, Gene Disruption Project members, 2001-, Zhang et al., 2001, Hummel et al., 2000, Roos et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Coyne et al., 2014)

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Coyne et al., 2014)

exacerbates Troyer syndrome

modeled by spartin¹

(Nahm et al., 2013)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 3

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Zhang et al., 2001, Roos et al., 2000)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

(Nahm et al., 2013)

abnormal neurophysiology, with Scer\GAL4^elav-C155

(Zhang et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Stage 16/17 embryos expressing futsch^EP1419 under the control of Scer\GAL4^elav-C155 do not show axonal guidance defects in the ventral nerve cord.

(Dinges et al., 2017)

Expression of futsch^EP1419 under the control of Scer\GAL4^D42 has no effect on NMJ bouton number, but does result in longer presynaptic terminals compared with controls.

(Coyne et al., 2014)

Expression of futsch^EP1419 under the control of Scer\GAL4^BG380 has no effect on maximum bouton diameter at the larval neuromuscular junction.

(Klinedinst et al., 2013)

Expression of futsch^EP1419 under the control of Scer\GAL4^elav-C155 results in vacuolisation and increased cell death in the brains of 20 day old flies.

(Nahm et al., 2013)

Expression of futsch^EP1419 pre-synaptically, under the control of Scer\GAL4^elav-C155 has no significant effect on synaptic mitochondria.

(Lee et al., 2010)

When expression is driven by Scer\GAL4^elav-C155 the phenotype is similar to that of Fmr1 loss of function mutants - neuromuscular junction overgrowth with increased synaptic area, branching and bouton number. Neurotransmission at the NMJ is elevated, and in the photoreceptor is depressed.

(Zhang et al., 2001)

When expression of futsch^EP1419 is driven by Scer\GAL4^sim.PS, changes in the morphology of individual growth cones is seen, and minor defects in the projection of individual axons are also seen. However these early axonal phenotypes do not lead to defects in the later formation of the central nervous system (CNS) axon pattern.

(Hummel et al., 2000)

When futsch^EP1419 is expressed under the control of Scer\GAL4^elav-C155, a slight reduction in average bouton size is seen (about 7.1μm², compared to 9.1μm² in wild-type).

(Roos et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, futsch^EP1419 has abnormal neuroanatomy | adult stage phenotype, enhanceable by spartin¹/Df(3R)110

(Nahm et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, futsch^EP1419 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/spartin^UAS.Tag:HA

(Nahm et al., 2013)

Enhancer of

Statement

Reference

futsch^EP1419/Scer\GAL4^ppk.PG is an enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is an enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

Suppressor of

Statement

Reference

futsch^EP1419/Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage | progressive phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of lola^F.znf

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of lola^e76

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of lola^F.stop

(Dinges et al., 2017)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal locomotor behavior | larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of short lived phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal locomotor behavior | larval stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of short lived phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of abnormal neuroanatomy phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

Scer\GAL4^BG380/futsch^EP1419 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Aplip1^ex

(Klinedinst et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, futsch^EP1419 has adult brain phenotype, enhanceable by spartin¹/Df(3R)110

(Nahm et al., 2013)

Scer\GAL4⁴⁴, futsch^EP1419 has axon phenotype, enhanceable by Rnor\Map2^UAS.cAa, Scer\GAL4⁴⁴

(Hummel et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, futsch^EP1419 has adult brain phenotype, suppressible | partially by Scer\GAL4^elav-C155/spartin^UAS.Tag:HA

(Nahm et al., 2013)

Enhancer of

Statement

Reference

futsch^EP1419/Scer\GAL4^ppk.PG is an enhancer of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

futsch^EP1419/Scer\GAL4^ppk.PG is an enhancer of dendrite | pupal stage phenotype of Scer\GAL4^ppk.PG, par-1^HMS00405

(Herzmann et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is an enhancer of adult brain | progressive phenotype of spartin¹/Df(3R)110

(Nahm et al., 2013)

futsch^EP1419, Scer\GAL4⁴⁴ is an enhancer of axon phenotype of Rnor\Map2^UAS.cAa, Scer\GAL4⁴⁴

(Hummel et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^RapGAP1-OK6/futsch^EP1419 is a suppressor of type Ib terminal bouton | increased number | larval stage phenotype of αTub84B^K394R

(Saunders et al., 2022)

Scer\GAL4^RapGAP1-OK6/futsch^EP1419 is a suppressor of microtubule | larval stage phenotype of αTub84B^K394R

(Saunders et al., 2022)

Scer\GAL4^RapGAP1-OK6/futsch^EP1419 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of αTub84B^K394R

(Saunders et al., 2022)

Scer\GAL4^RapGAP1-OK6/futsch^EP1419 is a suppressor of type Is terminal bouton | larval stage | decreased number phenotype of αTub84B^K394R

(Saunders et al., 2022)

futsch^EP1419/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of embryonic/larval neuromuscular junction | embryonic stage phenotype of lola^F.stop

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of axon | embryonic stage phenotype of lola^F.stop

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of ventral midline of embryo | embryonic stage phenotype of lola^F.stop

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of embryonic/larval neuromuscular junction | embryonic stage phenotype of lola^F.znf

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of axon | embryonic stage phenotype of lola^F.znf

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of ventral midline of embryo | embryonic stage phenotype of lola^F.znf

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of embryonic/larval neuromuscular junction | embryonic stage phenotype of lola^e76

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of axon | embryonic stage phenotype of lola^e76

(Dinges et al., 2017)

Scer\GAL4^elav-C155/futsch^EP1419 is a suppressor | partially of ventral midline of embryo | embryonic stage phenotype of lola^e76

(Dinges et al., 2017)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of NMJ bouton | larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

futsch^EP1419, Scer\GAL4^Toll-6-D42 is a suppressor of NMJ bouton phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Coyne et al., 2014)

Scer\GAL4^BG380/futsch^EP1419 is a suppressor of NMJ bouton | larval stage phenotype of Aplip1^ex

(Klinedinst et al., 2013)

futsch^EP1419, Scer\GAL4^elav-C155 is a suppressor of type I bouton phenotype of Hsap\LRRK2^UAS.Tag:FLAG, Scer\GAL4^elav-C155

(Lee et al., 2010)

futsch^EP1419, Scer\GAL4^elav-C155 is a suppressor of neuromuscular junction phenotype of Hsap\LRRK2^UAS.Tag:FLAG, Scer\GAL4^elav-C155

(Lee et al., 2010)

futsch^EP1419, Scer\GAL4^elav-C155 is a suppressor of mitochondrion phenotype of Hsap\LRRK2^UAS.Tag:FLAG, Scer\GAL4^elav-C155

(Lee et al., 2010)

NOT Suppressor of

Statement

Reference

futsch^EP1419, Scer\GAL4^Mhc.PW is a non-suppressor of type I bouton phenotype of Hsap\LRRK2^UAS.Tag:FLAG, Scer\GAL4^Mhc.PW

(Lee et al., 2010)

futsch^EP1419, Scer\GAL4^Mhc.PW is a non-suppressor of neuromuscular junction phenotype of Hsap\LRRK2^UAS.Tag:FLAG, Scer\GAL4^Mhc.PW

(Lee et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1^HMS00405 under the control of Scer\GAL4^ppk.PG is weakly enhanced by co-expression of futsch^EP1419.

(Herzmann et al., 2017)

Expression of futsch^EP1419 under the control of Scer\GAL4^BG380 completely suppresses the increase in maximum bouton diameter which is seen at the neuromuscular junction in Aplip1^ex larvae.

Expression of futsch^EP1419 under the control of Scer\GAL4^BG380 fails to suppress the defects in axonal transport seen in Aplip1^ex larvae.

(Klinedinst et al., 2013)

Expression of futsch^EP1419 under the control of Scer\GAL4^elav-C155 in a spartin¹/Df(3R)110 background enhances the number of vacuoles in the brain of 20 day old flies compared to either single mutant.

Co-expression of spartin^{Scer\UAS.T:Ivir\HA1} slightly but significantly suppresses the increased vacuolisation in the brain seen in 20 day old flies expressing futsch^EP1419 under the control of Scer\GAL4^elav-C155.

(Nahm et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of futsch^EP1419 partially suppresses the larval turning and reduced lifespan phenotypes seen when Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa} is expressed under the control of Scer\GAL4^D42.

Expression of futsch^EP1419 partially suppresses the larval turning and reduced lifespan phenotypes seen when Hsap\TARDBP^{G298S.Scer\UAS.T:Avic\GFP-YFP} is expressed under the control of Scer\GAL4^D42.

Expression of futsch^EP1419 fully suppresses the reduction in boutons compared with controls seen when Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa} is expressed under the control of Scer\GAL4^D42. The increase in satellite bouton number in the larval NMJ is suppressed to control levels.

Expression of futsch^EP1419 fully suppresses the reduction in boutons compared with controls seen when Hsap\TARDBP^{G298S.Scer\UAS.T:Avic\GFP-YFP} is expressed under the control of Scer\GAL4^D42. The increase in satellite bouton number in the larval NMJ is suppressed to control levels.

(Coyne et al., 2014)

Co-expression of futsch^EP1419 pre-synaptically rescues the synaptic overgrowth phenotype found upon expression of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG} pre-synaptically under the control of Scer\GAL4^elav-C155. However, synaptic defects caused by post-synaptic expression of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG} are not suppressed when futsch^EP1419 is expressed post-synaptically (under the control of Scer\GAL4^Mhc.PW).

The abnormal microtubule phenotype seen upon Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG} presynaptic expression is rescued by a futsch^EP1419 expression (both under the control of Scer\GAL4^elav-C155).

Neuronal co-expression of futsch^EP1419 with Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG} rescues the mitochondrial distribution phenotype seen upon Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG} expression alone.

(Lee et al., 2010)

Expression of futsch^EP1419 and Rnor\MAP2^Scer\UAS.cAa driven by Scer\GAL4⁴⁴ results in frequent axonal swellings and misprojection phenotypes. In 1/5 of neuromeres sensory neurons project their axons in on one of the two commissures across the central nervous system (CNS) midline and fasciculate with their contralateral counterparts, which they never do in wild-type.

(Hummel et al., 2000)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10751

P{EP}futsch^EP1419 w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP(1)1419

(Benos et al., 2001)

Futsch^EP(x)1419

(Klinedinst et al., 2013)

futsch^EP1419

(Saunders et al., 2022, Kankel et al., 2020, Shi et al., 2019, Dinges et al., 2017, Herzmann et al., 2017, Nahm et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (16)

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