FlyBase Allele Report: Dmel\kirre[UAS.cRa]

General Information

Symbol

Dmel\kirre^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ruiz-Gomez

FlyBase ID

FBal0118181

Feature type

allele

Associated gene

Dmel\kirre

Associated Insertion(s)

Carried in Construct

P{UAS-kirre.R}

Also Known As

UAS-Duf, UAS-kirre

Key Links

Transgenic product class

wild_type

(Ruiz-Gomez et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ruiz-Gomez et al., 2000)

Mutagen

in vitro construct

(Ruiz-Gomez et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-kirre.R}

Cytology

Description

UASt regulatory sequences drive expression of a 3.5kb XbaI-NotI fragment containing the entire kirre open reading frame.

(Ruiz-Gomez et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kirre

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | embryonic stage, with Scer\GAL4^GMR88F09

(Bertin et al., 2021)

visible, with Scer\GAL4^hs.2sev

(Helmstadter et al., 2012)

Phenotype Manifest In

adult optic lobe, with Scer\GAL4^MZ1369

(Helmstadter et al., 2012)

embryonic/larval garland cell | third instar larval stage, with Scer\GAL4^pros.PMG

(Helmstadter et al., 2012)

eye, with Scer\GAL4^hs.2sev

(Helmstadter et al., 2012)

lateral transverse muscle cell | embryonic stage | increased number, with Scer\GAL4^GMR88F09

(Bertin et al., 2021)

muscle cell of ventral acute muscle 2, with Scer\GAL4^kirre.PR

(Bataillé et al., 2010)

myoblast, with Df(1)w67k30, Scer\GAL4^Dll-md23

(Ruiz-Gomez et al., 2000)

myoblast, with Df(1)w67k30, Scer\GAL4^wg.PM

(Ruiz-Gomez et al., 2000)

nucleus | embryonic stage | increased number, with Scer\GAL4^GMR88F09

(Bertin et al., 2021)

nucleus | increased number, with Scer\GAL4^kirre.PR

(Bataillé et al., 2010)

nucleus | increased number | third instar larval stage, with Scer\GAL4^pros.PMG

(Helmstadter et al., 2012)

segment border muscle cell, with Scer\GAL4^kirre.PR

(Bataillé et al., 2010)

Detailed Description

Statement

Reference

Embryos expressing kirre^UAS.cRa under the control of Scer\GAL4^GMR88F09 show lateral transverse muscle defects: muscle splitting and increased number of nuclei.

(Bertin et al., 2021)

Wild-type garland cell nephrocytes (GCNs) fuse to form binucleate GCNs in third instar larvae. In contrast, overexpression of kirre^Scer\UAS.cRa driven by Scer\GAL4^pros.PMG results in excessive GCN fusion resulting in multinucleate GCN cells in third instar larvae.

Overexpression of kirre^Scer\UAS.cRa using Scer\GAL4^hs.2sev results in a rough eye phenotype.

Overexpression of kirre^Scer\UAS.cRa using Scer\GAL4^MZ1369 results in optic lobe abnormalities with variable penetrance.

(Helmstadter et al., 2012)

Overexpression of kirre^Scer\UAS.cRa using Scer\GAL4^kirre.PR leads to an increased number of nuclei in all muscles analysed (VA2 and SBM).

There is no change in the numbero of SBM nuclei upon expression of kirre^Scer\UAS.cRa using Scer\GAL4^slou.S59.

(Bataillé et al., 2010)

When kirre^Scer\UAS.cRa is expressed under the control of Scer\GAL4^Dll-md23 in a Df(1)w67k30 background, the migration of myoblasts is redirected toward sites of ectopic kirre expression. In these embryos, there is a substantial aggregation of myoblasts in the head and on the primordia of the leg discs. When kirre^Scer\UAS.cRa is expressed under the control of Scer\GAL4^wg.PM in a Df(1)w67k30 background, myoblasts migrate towards the site of ectopic kirre expression; unfused myoblasts distribute themselves on the inner face of the epidermis in a segmentally repated pattern of bands at stage 12 and myoblasts are also attracted toward a restricted region of the visceral mesoderm surrounding the midgut (parasegment 8), where wg is normally expressed.

(Ruiz-Gomez et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^sns.GCN/kirre^UAS.cRa rescues kirre^sps-1

(Helmstadter et al., 2012)

Scer\GAL4^how-24B/kirre^UAS.cRa rescues Df(1)w67k30

(Menon et al., 2005)

Partially rescues

Scer\GAL4^how-24B/kirre^UAS.cRa partially rescues Df(1)w67k30

(Menon et al., 2005)

Scer\GAL4^ap-md544/kirre^UAS.cRa partially rescues Df(1)w67k30

(Ruiz-Gomez et al., 2000)

Scer\GAL4^twi.PB/kirre^UAS.cRa partially rescues Df(1)w67k30

(Ruiz-Gomez et al., 2000)

Fails to rescue

kirre^UAS.cRa/Scer\GAL4^sns.PK fails to rescue Df(1)w67k30

(Haralalka et al., 2011)

Comments

The garland cell phenotype associated with kirre^sps-1 mutants is rescued by the expression of kirre^Scer\UAS.cRa using Scer\GAL4^sns.GCN.

(Helmstadter et al., 2012)

Expression of kirre^Scer\UAS.cRa under the control of Scer\GAL4^sns.PK does not rescue myoblast fusion in Df(1)w67k30 embryos.

(Haralalka et al., 2011)

Expression of kirre^Scer\UAS.cRa under the control of Scer\GAL4^how-24B restores the ability of myoblast founders to attract fusion-competent myoblasts in Df(1)w67k30 mutant embryos. Myoblast fusion occurs to a significant extent and a wild type muscle pattern in every segment is observed.

(Menon et al., 2005)

Expression of kirre^Scer\UAS.cRa under the control of Scer\GAL4^twi.PB restores fusion in every muscle of Df(1)w67k30 embryos. The muscle pattern is almost wild type in the rescued embryos, though with some smaller muscles and scattered unfused myoblasts. There is an accumulation of unfused myoblasts around the hindgut and at other locations where twi is normally expressed at high levels (these phenotypes may be a consequence of using Scer\GAL4^twi.PB as the driver). Expression of kirre^Scer\UAS.cRa under the control of Scer\GAL4^ap-md544 restores fusion exclusively in LT and VA muscles.

(Ruiz-Gomez et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kirre^Scer\UAS.cRa

kirre^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ruiz-Gomez

(Ruiz-Gomez et al., 2000)

Secondary FlyBase IDs

References (9)

Report Sections

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