FB2024_03 , released June 25, 2024
Allele: Dmel\mei-P26mfs1
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General Information
Symbol
Dmel\mei-P26mfs1
Species
D. melanogaster
Name
FlyBase ID
FBal0104481
Feature type
allele
Associated gene
Dmel\mei-P26
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Page et al., 2000)
Progenitor genotype
mei-P261
(Page et al., 2000)
Cytology
Description

Deletion of the ends of the P{lacW} element and 0.7-1.6kb of DNA flanking both sides of the original insertion site. There may also be a more complex rearrangement, possibly involving the insertion of sequence. Some P{lacW} sequence may remain.

(Page et al., 2000)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mei-P26mfs1 ovaries exhibit arrest in early oogenesis.

      (Avilés-Pagán et al., 2020)

      Testes of homozygous males have cysts full of many small cells, which appear similar to early germ cells seen at the apical tip of the wild-type testis. EdU incorporation assays show that early germ cells in the mutant testis undergo several extra rounds of mitotic division and behave as transit amplifying cells rather than as stem cells, with all cells within a cyst undergoing S phase synchronously. The overproliferating cysts in the mutant testes eventually degenerate. The nucleoli of mutant early germ cells are larger than normal. 32% of germline cysts do switch to the spermatocyte state, but only a third of these contain the normal number of 16 spermatocytes per cyst and the differentiating cells show a number of gross morphological defects.

      (Insco et al., 2012)

      Germaria are filled with individual spectrosome-containing cells and cysts containing varying numbers of cells connected by a fusome in mutant ovaries. Nurse cells and oocytes are not formed and fusomes or spectrosomes are maintained at later stages of oogenesis.

      Cell diameters are increased compared to wild type and no longer decrease as cells are displaced from the stem cell niche in mei-P26fs1/mei-P26mfs1 ovaries.

      (Neumuller et al., 2008)

      Homozygous ovaries are completely tumourous and no normal looking egg chambers are seen. Homozygous females do not lay eggs. Testes of hemizygous males do not contain any mature motile spermatozoa, although somewhat disorganised immature elongated spermatid bundles are produced. In addition, the testes contain highly refractile cysts that most likely represent degenerating cysts of spermatogonial cells.

      (Page et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      mei-P26mfs1 has male germline cyst phenotype, suppressible | partially by bam+t2.9

      (Insco et al., 2012)

      mei-P26mfs1 has male germline cyst phenotype, suppressible | partially by bamΔPEST

      (Insco et al., 2012)

      mei-P26mfs1 has nucleolus phenotype, suppressible by bam+t2.9

      (Insco et al., 2012)

      mei-P26mfs1 has nucleolus phenotype, suppressible by bamΔPEST

      (Insco et al., 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of bam+t2.9 completely rescues the overproliferation defects seen in the testes of mei-P26mfs1 males and 72% of the cysts in the spermatocyte region have 16 spermatocytes per cyst. The enlarged nucleolus phenotype seen in mei-P26mfs1 testes is also suppressed. The spermatocyte and spermatid differentiation defects are not rescued: 27% of cysts from the spermatocyte region have differentiation defects in the rescued males.

      Expression of bamΔPEST completely rescues the overproliferation defects seen in the testes of mei-P26mfs1 males and 43% of the cysts in the spermatocyte region have 16 spermatocytes per cyst. The enlarged nucleolus phenotype seen in mei-P26mfs1 testes is also suppressed. The spermatocyte and spermatid differentiation defects are not rescued: 30% of cysts from the spermatocyte region have differentiation defects in the rescued males.

      (Insco et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      mei-P261

      (Page et al., 2000)
      Rescued by

      mei-P26mfs1 is rescued by mei-P26+t13

      (Insco et al., 2012)

      mei-P26mfs1 is rescued by mei-P26+t13

      (Page et al., 2000)
      Comments

      The overproliferating spermatogonial cysts and spermatocyte differentiation defects seen in the testes of mei-P26mfs1 males are rescued by Dp(1;4)A17 and by mei-P26+t13.

      (Insco et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (12)