FlyBase Allele Report: Dmel\osa[RD.UAS]

General Information

Symbol

Dmel\osa^RD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0103836

Feature type

allele

Associated gene

Dmel\osa

Associated Insertion(s)

Carried in Construct

P{UAS-osaRD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Collins et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-osaRD}

Cytology

Description

453bp of osa 5'UTR and the first 7 amino acids of osa are followed by a fragment of the osa cDNA encoding the ARID DNA binding domain (amino acids 965-1198). This is fused to the repressor domain of en (amino acids 168-282). Expression is under the control of UASt regulatory sequences.

(Collins et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

osa

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^bi-omb-Gal4

(Collins et al., 1999)

visible, with Scer\GAL4^pnr-MD237

(Collins et al., 1999)

Phenotype Manifest In

macrochaeta | ectopic, with Scer\GAL4^pnr-MD237

(Collins et al., 1999)

microchaeta | ectopic, with Scer\GAL4^pnr-MD237

(Collins et al., 1999)

wing margin bristle | ectopic, with Scer\GAL4^bi-omb-Gal4

(Collins et al., 1999)

wing vein, with Scer\GAL4^bi-omb-Gal4

(Collins et al., 1999)

Detailed Description

Statement

Reference

Expression of en::osa^{osaRD.Scer\UAS} in the notum driven by Scer\GAL4^pnr-MD237 induces the formation of ectopic macrochaetae and microchaetae. Expression of en::osa^{osaRD.Scer\UAS} in the notum driven by Scer\GAL4^bi-omb-Gal4 induces the formation of ectopic wing margin bristles and a disruption of wing vein morphology.

(Collins et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^bbg-C96/osa^RD.UAS is a non-enhancer of visible phenotype of ct^K

(Krupp et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^bbg-C96/osa^RD.UAS is a suppressor of visible phenotype of ct^53d

(Krupp et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^bbg-C96/osa^RD.UAS is a non-suppressor of visible phenotype of ct^K

(Krupp et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^bi-omb-Gal4, osa^RD.UAS has wing vein phenotype, enhanceable by osa³⁰⁸

(Collins et al., 1999)

Scer\GAL4^bi-omb-Gal4, osa^RD.UAS has wing margin bristle | ectopic phenotype, enhanceable by osa³⁰⁸

(Collins et al., 1999)

NOT Enhancer of

Statement

Reference

Scer\GAL4^bbg-C96/osa^RD.UAS is a non-enhancer of triple row of wing sensilla phenotype of ct^K

(Krupp et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^bbg-C96/osa^RD.UAS is a suppressor of wing phenotype of ct^53d

(Krupp et al., 2005)

Scer\GAL4^pnr-MD237/osa^RD.UAS is a suppressor of macrochaeta phenotype of Scer\GAL4^pnr-MD237, osa^VP16.AD.UAS

(Collins et al., 1999)

NOT Suppressor of

Statement

Reference

Scer\GAL4^bbg-C96/osa^RD.UAS is a non-suppressor of triple row of wing sensilla phenotype of ct^K

(Krupp et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of en::osa^{osaRD.Scer\UAS} suppresses the loss of macrochaetae seen in osa^{AD.Scer\UAS.T:Hsim\VP16}/Scer\GAL4^pnr-MD237 flies. The phenotype caused by expression of en::osa^{osaRD.Scer\UAS} in the notum driven by Scer\GAL4^bi-omb-Gal4 is enhanced by the addition of osa³⁰⁸.

(Collins et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

en::osa^{osaRD.Scer\UAS}

osa^RD.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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