FlyBase Allele Report: Dmel\hh[N.UAS.Tag:HA]

General Information

Symbol

Dmel\hh^N.UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0102695

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

P{UAS-hh.N.HA}

Also Known As

UAS-Hh-N, UAS-hh-N^HA

Key Links

Transgenic product class

stop_gained

(Burke et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Burke et al., 1999)

Mutagen

in vitro construct

(Burke et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-hh.N.HA}

Cytology

Description

UASt regulatory sequences drive expression of the N-terminal portion of hh (a stop codon follows residue G257). A triple Tag:HA tag is inserted between hh amino acid residues 254 and 255.

(Burke et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

photoreceptor neuron, with Scer\GAL4^ey.PH

(Lee and Treisman, 2001)

wing disc anterior compartment, with Scer\GAL4^en-e16E

(Burke et al., 1999)

wing disc anterior compartment, with Scer\GAL4^en-e16E, hh^ts2

(Chamoun et al., 2001)

Detailed Description

Statement

Reference

Expression of hh^{N.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E in a hh^ts2 background under nonpermissive conditions results in overgrowth of the anterior compartment of the wing disc.

(Chamoun et al., 2001)

hh^{N.Scer\UAS.T:Ivir\HA1} when driven by Scer\GAL4^ey.PH causes premature photoreceptor differentiation in wild-type eye discs.

(Lee and Treisman, 2001)

Expression of hh^{N.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E results in a dramatic enlargement of the anterior compartment of the wing disc.

(Burke et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, hh^N.UAS.Tag:HA has wing disc | anterior phenotype, non-suppressible by disp^S037707

(Burke et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth of the anterior compartment of the wing disc seen in larvae expressing hh^{N.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E in a hh^ts2 background is still seen if the discs are also carrying disp^S037707.

(Burke et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

hh^N.UAS.Tag:HA/Scer\GAL4^GMR.PF rescues hh^bar3

(Tokhunts et al., 2010)

hh^N.UAS.Tag:HA/Scer\GAL4^en-e16E rescues hh^ts2

(Burke et al., 1999)

Comments

Rescues the growth defects of hh^ts2 wing discs when expressed under the control of Scer\GAL4^en-e16E (anterior outgrowth of the rescued discs is seen, as in discs expressing hh^{N.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E in a hh⁺ background).

(Burke et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hh^{N.Scer\UAS.T:Ivir\HA1}

hh^N.UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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