FlyBase Allele Report: Zzzz\aptamer[B52.BBS.UAS]

General Information

Symbol

Zzzz\aptamer^B52.BBS.UAS

Species

a. artificial

Name

FlyBase ID

FBal0101524

Feature type

allele

Associated gene

Zzzz\aptamer

Associated Insertion(s)

Carried in Construct

P{UAS-B52.BBS}

Also Known As

UAS-BBS

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Shi et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-B52.BBS}

Cytology

Description

Expression of an aptamer RNA that binds B52 protein is governed by UASt regulatory sequences. The iaRNA gene includes two tandemly repeated pentameric aptameric units. Each unit contains five aptamers which form stem loop structures, followed by a stable tetra loop, followed by a hammer head ribozyme.

(Shi et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\aptamer

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | embryonic stage, with Scer\GAL4^elav.PLu

(Liu and Bossing, 2016)

abnormal locomotor behavior | first instar larval stage, with Scer\GAL4^elav.PLu

(Liu and Bossing, 2016)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^CY27

(Liu and Bossing, 2016)

partially lethal - majority die, with Scer\GAL4^hs.PB

(Shi et al., 1999)

Phenotype Manifest In

dMP2 neuron | embryonic stage, with Scer\GAL4^CY27

(Liu and Bossing, 2016)

Detailed Description

Statement

Reference

Expression of Zzzz\aptamer^{B52.BBS.Scer\UAS} under the control of Scer\GAL4^elav.PLu significantly delays larval hatching from embryos and delays the maturation of larval muscle movement: the larvae show an increase in the frequency of uncoordinated muscle twitches that occur at early larval stage (before tracheal filling) before being gradually replaced by coordinated muscle contractions. Expression under the Scer\GAL4^eg-Mz360 driver does not impair neuronal cell morphology or number, neither does it interfere with axon guidance or neuronal determination and cell division. Expression of Zzzz\aptamer^{B52.BBS.Scer\UAS} controlled by the Scer\GAL4^CY27 driver increases axon extension in embryonic dMP2 neurons.

(Liu and Bossing, 2016)

Flies carrying P{UAS-B52.BBS} show a reduction in viability, even in the absence of Scer\GAL4^hs.PB. When expression is governed by Scer\GAL4^hs.PB, heat induction causes up to a 50% reduction in survival though surviving flies are morphologically normal. When expression is driven by Scer\GAL4^dpp.blk1 salivary glands and wings are normal. When expression is driven by Scer\GAL4^G-17 abdominal sternites are normal. When expression is driven by Scer\GAL4^I-65 more than 90% of progeny class survive.

(Shi et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Zzzz\aptamer^B52.BBS.UAS, Scer\GAL4^hs.PB is a suppressor | partially of visible phenotype of B52^UAS.cKa, Scer\GAL4^hs.PB

(Shi et al., 1999)

Zzzz\aptamer^B52.BBS.UAS, Scer\GAL4^dpp.blk1 is a suppressor | partially of visible phenotype of B52^UAS.cKa, Scer\GAL4^dpp.blk1