FlyBase Allele Report: Dmel\Pi3K21B[UAS.Δ.Tag:HA]

General Information

Symbol

Dmel\Pi3K21B^{UAS.Δ.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0100421

Feature type

allele

Associated gene

Dmel\Pi3K21B

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K21BΔ.HA}

Also Known As

UAS-Δp60

Key Links

Transgenic product class

inframe_deletion

(Weinkove et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Weinkove et al., 1999)

Mutagen

in vitro construct

(Weinkove et al., 1999)

Progenitor genotype

Pi3K21B^UAS.Tag:HA

(Weinkove et al., 1999)

Carried in construct

P{UAS-Pi3K21BΔ.HA}

Cytology

Description

Deletion of amino acids 466-558.

(Weinkove et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pi3K21B

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002, Martin-Castellanos and Edgar, 2002)

Phenotype Manifest In

cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

(Martin-Castellanos and Edgar, 2002)

embryonic/larval fat body | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^bs-23.26

(Gallio et al., 2004)

embryonic/larval salivary gland | somatic clone, with Scer\GAL4^Act5C.PP

(Britton et al., 2002)

imaginal disc & cell, with Scer\GAL4^Act5C.PP

(Weinkove et al., 1999)

Detailed Description

Statement

Reference

Migration of about a fifth of ganglionic tracheal branches stalls in stage 16 Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}; Scer\GAL4^bs-23.26 embryos.

(Gallio et al., 2004)

Caspase activation in cells posterior to the furrow in eye discs is only slightly reduced by Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF.

(Yang and Baker, 2003)

Expression of Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP or Scer\GAL4^Adh.PF inhibits larval growth. Clones of cells expressing Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP in the salivary glands and fat body are greatly reduced in size and have much smaller nuclei with far less DNA than adjacent control cells. Expression of Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} under the control of Scer\GAL4^Adh.PF, Scer\GAL4^en-e16E or Scer\GAL4^Act5C.PP has no effect on viability under starvation conditions.

(Britton et al., 2002)

Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} clones in the wing disc (driven by Scer\GAL4^Act5C.PP) show a reduced cell size and delays in G1 progression. Clones have very few cells compared to controls.

(Martin-Castellanos and Edgar, 2002)

When expression is driven is imaginal disc cells by Scer\GAL4^Act5C.PP the size of the cells is reduced (as measured by FSC (forward scatter)). Cells are reduced in size in both G1 and S+G2. Overexpression of Pi3K21B^{Scer\UAS.T:Ivir\HA1} or Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} is sufficient to reduce the rate of increase of cell number.

(Weinkove et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pi3K21B^{UAS.Δ.Tag:HA}, Scer\GAL4^Act5C.PP is a suppressor of increased cell number | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Pi3K21B^{UAS.Δ.Tag:HA}, Scer\GAL4^Act5C.PP is a suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Other

Statement

Reference

Pi3K21B^{UAS.Δ.Tag:HA}, Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc} has increased cell number | somatic clone phenotype

(Martin-Castellanos and Edgar, 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pi3K21B^{UAS.Δ.Tag:HA}, Scer\GAL4^Act5C.PP has cell cycle | somatic clone phenotype, suppressible by tkv^{Q253D.UAS.cNc}, Scer\GAL4^Act5C.PP

(Martin-Castellanos and Edgar, 2002)

Suppressor of

Statement

Reference

Pi3K21B^{UAS.Δ.Tag:HA}, Scer\GAL4^Act5C.PP is a suppressor of cell cycle | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of tkv^{Q253D.Scer\UAS.cNc} to Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} (driven by Scer\GAL4^Act5C.PP) suppresses the G1 delay phenotype seen in Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}, Scer\GAL4^Act5C.PP clones alone.

(Martin-Castellanos and Edgar, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}

Pi3K21B^{UAS.Δ.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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