FB2024_03 , released June 25, 2024
Allele: Dmel\UbxIa.VP16.UAS
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General Information
Symbol
Dmel\UbxIa.VP16.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0098788
Feature type
allele
Associated gene
Dmel\Ubx
Associated Insertion(s)
Carried in Construct
P{UAS-Ubx-VP16}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Li and McGinnis, 1999)
Progenitor genotype
Carried in construct
P{UAS-Ubx-VP16}
Cytology
Description

UASt regulatory sequences drive expression of the Ia isoform of Ubx fused at its C-terminal end to the Hsim\VP16 activation domain.

(Li and McGinnis, 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ubx
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When ectopically expressed by a strong Scer\GAL4arm.PS line (arm-GAL4), UbxIa.Scer\UAS.T:Hsim\VP16 generates a range of phenotypes. Individual denticle morphologies in T2 and T3 appear normal though the numbers of denticles are reduced. T1 develops without the characteristic patch of beard denticles. In contrast to ectopic UbxScer\UAS.cCa, the remaining T1 denticles have a T2-like morphology. The head segments fail to involute and remain external and T2-like denticles are generated ectopically in these head segments. High doses of UbxIa.Scer\UAS.T:Hsim\VP16 can also suppress the formation of the Keilin's organs. When ectopically expressed by a weaker Scer\GAL4arm.PS line (arm-GAL4r), UbxIa.Scer\UAS.T:Hsim\VP16 still retains the ability to specify T2-like denticles. T2 and T3 denticle belts remain wild type, T1 beard denticles are reduced, and the remaining T1 denticles at the anterior border of the segment are fewer and smaller. Some of these denticles in T1 now resemble T2 denticles. The partially involuted head segments develop ectopic patches of T2-like denticles. This phenotype of UbxIa.Scer\UAS.T:Hsim\VP16 driven by Scer\GAL4arm.PS resembles that of Antphs.PHTA.

      (Li and McGinnis, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      UbxIa.Scer\UAS.T:Hsim\VP16 driven by a weak expressing line of Scer\GAL4arm.PS (arm-GAL4r), in a Antp25 mutant background, displays a normal Antp loss of function phenotype. When driven by a strong expressing line of Scer\GAL4arm.PS (arm-GAL4), the Antp25 is rescued almost to wild type; T2 and T3 are rescued to their normal appearances, though some head segments develop small patches of T2-like denticles.

      (Li and McGinnis, 1999)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      UbxIa.Scer\UAS.T:Hsim\VP16
      UbxIa.VP16.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)