FB2024_03 , released June 25, 2024
Allele: Dmel\Egfr1.UAS
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General Information
Symbol
Dmel\Egfr1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0095742
Feature type
allele
Associated gene
Dmel\Egfr
Associated Insertion(s)
Carried in Construct
P{Egfr.1.UAS}
Also Known As
UAS-Egfr type I
Key Links
Transgenic product class
wild_type
(Lesokhin et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Lesokhin et al., 1999)
Mutagen
in vitro construct
(Lesokhin et al., 1999)
Progenitor genotype
Carried in construct
P{Egfr.1.UAS}
Cytology
Description

Expression of wild type Type 1 Egfr coding region is governed by UASt regulatory sequences.

(Lesokhin et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Egfr
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Egfr1.Scer\UAS under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B) results in an increase in intestinal stem cell division.

      (Huang et al., 2014)

      The number of cells posterior to the furrow in eye discs that display caspase activation is reduced in Egfr1.Scer\UAS; Scer\GAL4GMR.PF animals (Note: this effect is less dramatic than that seen with Egfr2.Scer\UAS). There is no accompanying increase in the levels of photoreceptor differentiation at this stage, but there is an increase in mitosis during the second mitotic wave (G2 to M progression, posterior to the furrow). The number of cells displaying caspase activation in pupal eye discs at 30 hours after puparium formation, is also significantly reduced in these animals, accompanied by significantly increased photoreceptor differentiation.

      (Yang and Baker, 2003)

      Expression of Egfr1.Scer\UAS under the control of Scer\GAL4GMR.PF results in division of all second mitotic wave cells in the eye. There is an increase in the mitotic index and mitoses are shifted more anteriorly. There is no increase in photoreceptor differentiation.

      (Baker and Yu, 2001)

      When expression is driven by Scer\GAL432B there is no effect on wing development. When expression is driven by Scer\GAL4h-H10 there is no effect on eye development.

      (Lesokhin et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of bansponge.Scer\UAS.T:Disc\RFP does not suppress the increase in intestinal stem cell division when Egfr1.Scer\UAS is expressed under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B).

      (Huang et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      Expression of Egfr1.Scer\UAS under the control of Scer\GAL4GMR.PF in EgfrE3 flies results in mitosis in all cells in the eye disc.

      (Baker and Yu, 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid " pUAST:dEGFR1 " and transfected into S3 cells.

      (Klueg et al., 2002)

      Carried in a plasmid, transfected into S3 cells and used in co-immunoprecipitation assays.

      (Alvarado et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Egfr1.Scer\UAS
      Egfr1.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (9)