FB2024_03 , released June 25, 2024
Allele: Dmel\Rbfhs.PD
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General Information
Symbol
Dmel\Rbfhs.PD
Species
D. melanogaster
Name
heat shock construct of Du
FlyBase ID
FBal0095616
Feature type
allele
Associated gene
Dmel\Rbf
Associated Insertion(s)
Carried in Construct
P{hs-Rbf.D}
Key Links
Transgenic product class
cDNA
(Du and Dyson, 1999)
wild_type
(Du and Dyson, 1999)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Du and Dyson, 1999)
wild_type
(Du and Dyson, 1999)
Mutagen
in vitro construct
(Du and Dyson, 1999)
Progenitor genotype
Carried in construct
P{hs-Rbf.D}
Cytology
Description

A full length Rbf cDNA is expressed under the control of a heat shock promoter.

(Du and Dyson, 1999)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Rbf
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult Rbf14 flies rescued by expressing Rbfhs.PD during the first 3 days after egg laying show a number of alterations in adult structures such as abnormal macrochaetae on the notum and rough eyes. All these adult phenotypes are rescued when Rbf function is provided throughout development using heat shock to express Rbfhs.PD. These adult phenotypes are also rescued without heat shock if the flies are also carrying E2f91/E2f164.

      (Du, 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Rbfhs.PD rescues Rbf14

      (Du, 2000)

      Rbfhs.PD rescues Rbf14/Rbf11

      (Du and Dyson, 1999)

      Rbfhs.PD rescues Rbf11

      (Du and Dyson, 1999)

      Rbfhs.PD rescues Rbf14

      (Du and Dyson, 1999)
      Comments

      Expression of Rbfhs.PD using heat shock can rescue the lethality of Rbf14 flies. One or two heat pulses of heat shock in the first 2 days after egg laying (AEL) is sufficient to rescue a small percentage of flies, while complete rescue of lethality is achieved by expression of Rbfhs.PD during the first 3 days AEL.

      (Du, 2000)

      Rescues the lethality of Rbf11/Rbf14, Rbf11/Y and Rbf14/Y flies.

      (Du and Dyson, 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rbfhs.PD
      Name Synonyms
      heat shock construct of Du
      (Du and Dyson, 1999)
      Secondary FlyBase IDs
        References (3)