FlyBase Allele Report: Dmel\N[ECNΔ10-12.UAS]

General Information

Symbol

Dmel\N^{ECNΔ10-12.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0094667

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.ECNΔ10-12}

Also Known As

UASECNΔ10-12

Key Links

Transgenic product class

inframe_deletion

(Jacobsen et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Jacobsen et al., 1998)

Mutagen

in vitro construct

(Brennan et al., 1999, Jacobsen et al., 1998)

Progenitor genotype

N^ECN.UAS

(Brennan et al., 1999, Jacobsen et al., 1998)

Carried in construct

P{UAS-N.ECNΔ10-12}

Cytology

Description

Deletion of N amino acids 412 to 566 inclusive, fusing EGF-like repeats 9 and 14, while maintaining the structural integrity of the remaining EGF-like repeats.

(Jacobsen et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000, Brennan et al., 1999)

visible, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

Phenotype Manifest In

macrochaeta, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

proneural cluster & dorsal mesothoracic disc, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

wing, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing disc, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing margin bristle, with Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

wing vein L3, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

wing vein L4, with Scer\GAL4^ptc-559.1

(Lawrence et al., 2000)

Detailed Description

Statement

Reference

Expression of N^{ECNΔ10-12.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 results in a fusion between wing veins 3 and 4.

(Lawrence et al., 2000)

When expression of N^{ECNΔ10-12.Scer\UAS} is driven by Scer\GAL4^sd-SG29.1 the wing disc has a small wing pouch. Adult wings lack the majority of both the anterior and posterior wing margin.

(Brennan et al., 1999)

Expression of N^{ECNΔ10-12.Scer\UAS} driven by Scer\GAL4^ptc-559.1 results in a reduction in cell number in those proneural clusters in the wing disc that are fated to become scutellar bristles, but not those fated to become dorsocentral bristles.

(Brennan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 has visible phenotype, non-enhanceable by Su(H)⁸/Su(H)²

(Brennan et al., 1999)

NOT suppressed by

Statement

Reference

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 has visible phenotype, non-suppressible by Su(H)⁸/Su(H)²

(Brennan et al., 1999)

Suppressor of

Statement

Reference

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a suppressor | partially of visible phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/N^{ECNΔ10-12.UAS} is a non-suppressor of visible phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^{ECNΔ10-12.UAS}, Scer\GAL4^sd-SG29.1 has wing disc phenotype, suppressible | partially by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

N^{ECNΔ10-12.UAS}, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible | partially by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Brennan et al., 1999)

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 has proneural cluster & dorsal mesothoracic disc phenotype, suppressible | somatic clone by sgg^M11

(Brennan et al., 1999)

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 has macrochaeta phenotype, suppressible | somatic clone by sgg^M11

(Brennan et al., 1999)

Suppressor of

Statement

Reference

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 is a suppressor of wing margin | ectopic phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

N^{ECNΔ10-12.UAS}, Scer\GAL4^ptc-559.1 is a suppressor of wing phenotype of Scer\GAL4^ptc-559.1, Ser^UAS.cSa

(Lawrence et al., 2000)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a suppressor | partially of anterior notopleural bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a suppressor | partially of posterior postalar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a suppressor | partially of posterior dorsocentral bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/N^{ECNΔ10-12.UAS} is a non-suppressor of wing margin | ectopic phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

Scer\GAL4^ptc-559.1/N^{ECNΔ10-12.UAS} is a non-suppressor of wing phenotype of Delta^UAS.cHa

(Lawrence et al., 2000)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a non-suppressor of presutural bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a non-suppressor of anterior supraalar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a non-suppressor of anterior postalar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a non-suppressor of anterior scutellar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

N^{ECNΔ10-12.UAS}, Scer\GAL4^{l(3)31-1-31-1} is a non-suppressor of posterior scutellar bristle & trichogen cell phenotype of Delta^UAS.cJa, Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

Other

Statement

Reference

N^{ECNΔ10-12.UAS}, Scer\GAL4^sd-SG29.1, wg^UAS.cGa has sensory mother cell & dorsal mesothoracic disc | ectopic phenotype

(Brennan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The wing phenotype caused by expression of Ser^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 is suppressed by coexpression of N^{ECNΔ10-12.Scer\UAS}. The wing phenotype caused by expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 is not suppressed by coexpression of N^{ECNΔ10-12.Scer\UAS}.

(Lawrence et al., 2000)

The addition of wg^{l-12.Scer\UAS} restores the wing pouch and adult wing almost to wild-type in animals expressing N^{ECNΔ10-12.Scer\UAS} driven by Scer\GAL4^sd-SG29.1. When expression of N^{ECNΔ10-12.Scer\UAS} and wg^Scer\UAS.cGa are driven by Scer\GAL4^sd-SG29.1 the whole of the anterior compartment of the wing disc is covered in evenly spaced SOPs (sensory organ precursors).

(Brennan et al., 1999)

The addition of Su(H)⁸/Su(H)² does not affect the phenotype caused by expression of N^{ECNΔ10-12.Scer\UAS} driven by Scer\GAL4^ptc-559.1. The loss of proneural precursors and sensory organ phenotypes normally seen in N^{ECNΔ10-12.Scer\UAS}/Scer\GAL4^ptc-559.1 flies is suppressed in sgg^M11 somatic clones.

(Brennan et al., 1999)

Coexpression of N^{ECNΔ10-12.Scer\UAS} fails to suppress the transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Dl^Scer\UAS.cJa expressed under the control of Scer\GAL4^{l(3)31-1-31-1} in the presutural, anterior supraalar, anterior postalar, anterior scutellar and posterior scutellar bristles. Transformation of the trichogen to a tormogen fate in the anterior notopleural, posterior postalar and posterior dorsocentral bristles is slightly reduced in these flies.

(Jacobsen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

N^{ECNΔ10-12.Scer\UAS}

N^{ECNΔ10-12.UAS}

Name Synonyms

Secondary FlyBase IDs

References (4)

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