A ci cDNA tagged at the N terminus with Tag:HA is expressed under the control of an αTub84B promoter.
visible
wing vein
Flies expressing ciαTub84B.T:Ivir\HA1 have wing vein defects but never have wing margin defects.
ciαTub84B.Tag:HA partially rescues ci94
Rescues ci94 to the pupal stage, with rare adult escapers.
N. Methot
Carried in a plasmid, transfected into Kc167 cells and assayed for cleavage of the ci protein.
Carried in a plasmid, transfected into Kc167 cells and assayed for cleavage of the ci protein.