EcollacZrn-89 was first detectable in the gap region between the B-H1 and dac domains as it forms at the beginning of the third instar. Prepupal legs show expression from presumptive tarsal segment 4 to the distal side of the presumptive metatarsus.
In third instar thoracic discs, highly expressed in central region of the discs, in regions corresponding to distal structures; no expression observed in eye disc.