FlyBase Allele Report: Dmel\tkv[1ΔGSK.UAS]

General Information

Symbol

Dmel\tkv^1ΔGSK.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0092484

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv.1ΔGSK}

Also Known As

UAS-tkv^DN, UAS-TkvDN, UAS-tkv1ΔGSK, UAS-tkv^DGSK

Key Links

Transgenic product class

dominant_negative_variant

(Haerry et al., 1998)

inframe_deletion

(Haerry et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Haerry et al., 1998)

inframe_deletion

(Haerry et al., 1998)

Mutagen

in vitro construct

(Haerry et al., 1998, Nguyen et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-tkv.1ΔGSK}

Cytology

Description

A dominant negative form of the tkv1 isoform, in which the GS box and kinase domain are deleted, is expressed under the control of UAS regulatory sequences.

(Haerry et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tkv

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

visible, with Scer\GAL4^ptc-559.1

(Crozatier et al., 2002)

Phenotype Manifest In

air sac primordium | third instar larval stage, with Scer\GAL4^btl.PS

(Roy et al., 2014)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Crozatier et al., 2002)

crossvein, with Scer\GAL4^A9

(Haerry et al., 1998)

dorsal row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^btl.PS

(Steneberg et al., 1999)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^esg-p127

(Steneberg et al., 1999)

medial row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

ventral row of triple row of wing sensilla, with Scer\GAL4^A9

(Haerry et al., 1998)

wing, with Scer\GAL4^A9

(Haerry et al., 1998)

wing, with Scer\GAL4^ptc-559.1

(Crozatier et al., 2002)

wing disc, with Scer\GAL4^A9

(Haerry et al., 1998)

wing vein L2, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

wing vein L3, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

wing vein L4, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

wing vein L4, with Scer\GAL4^ptc-559.1

(Crozatier et al., 2002)

wing vein L5, with Scer\GAL4^A9

(Haerry et al., 1998)

Detailed Description

Statement

Reference

Expression of tkv^{1ΔGSK.Scer\UAS} in trachea generates bifurcated, abnormally shaped air sac primordiums.

(Roy et al., 2014)

Expression of tkv^{1ΔGSK.Scer\UAS} under the control of Scer\GAL4^OK6, does not result in ISNb or SNa pathfinding defects.

(Parker et al., 2006)

Flies expressing tkv^{1ΔGSK.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 have smaller wings than normal with a loss of wing vein L4 and the anterior crossvein.

(Crozatier et al., 2002)

When expression is driven by Scer\GAL4^p127 in the fusion cells disrupts fusion in 19% of the dorsal branches. Suppression of expression of hdc suggests a failure of fusion cell differentiation. When expression is driven by Scer\GAL4^btl.PS a similar, though milder, phenotype results.

(Steneberg et al., 1999)

Flies expressing tkv^{1ΔGSK.Scer\UAS} (carrying 3 to 4 copies of P{UAS-tkv.1ΔGSK}) under the control of Scer\GAL4^A9 have small wings with partial loss of vein L4 and both cross veins. Veins L2 and L3 are closer together than normal and the triple row margin bristles are shifter more distally/posteriorly. Flies expressing higher levels of tkv^{1ΔGSK.Scer\UAS} (carrying 6 to 8 copies of P{UAS-tkv.1ΔGSK}) under the control of Scer\GAL4^A9 have very small wings that show fusion of veins L2 and L3 as well as L4 and L5. Wing discs of larvae expressing tkv^{1ΔGSK.Scer\UAS} under the control of Scer\GAL4^A9 are strongly reduced in size.

(Haerry et al., 1998)

Flies expressing two copies of tkv^{1ΔGSK.Scer\UAS} under the control of Scer\GAL4^A9 show partial loss of vein L4, and vein L2 starts to merge with L3.

(Nguyen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^sca.PU/tkv^1ΔGSK.UAS is a suppressor of visible phenotype of Scer\GAL4^sca.PU, Vap33^UAS.cRa

(Ratnaparkhi et al., 2008)

Scer\GAL4^A9/tkv^1ΔGSK.UAS is a suppressor | partially of visible phenotype of Scer\GAL4^A9, gbb^UAS.cKa

(Haerry et al., 1998)

Scer\GAL4^A9/tkv^1ΔGSK.UAS is a suppressor | partially of visible phenotype of Scer\GAL4^A9, dpp^UAS.cHa

(Haerry et al., 1998, Nguyen et al., 1998)

tkv^1ΔGSK.UAS is a suppressor | partially of visible phenotype of Scer\GAL4^A9, scw^UAS.cNa

(Nguyen et al., 1998)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^sca.PU/tkv^1ΔGSK.UAS is a suppressor of macrochaeta phenotype of Scer\GAL4^sca.PU, Vap33^UAS.cRa

(Ratnaparkhi et al., 2008)

Scer\GAL4^A9/tkv^1ΔGSK.UAS is a suppressor | partially of wing phenotype of Scer\GAL4^A9, gbb^UAS.cKa

(Haerry et al., 1998)

Scer\GAL4^A9/tkv^1ΔGSK.UAS is a suppressor | partially of wing phenotype of Scer\GAL4^A9, dpp^UAS.cHa

(Haerry et al., 1998, Nguyen et al., 1998)

tkv^1ΔGSK.UAS is a suppressor | partially of wing phenotype of Scer\GAL4^A9, scw^UAS.cNa

(Nguyen et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tkv^{1ΔGSK.Scer\UAS} partially suppresses the loss of thoracic bristles seen in adults expressing Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^sca.PU.

(Ratnaparkhi et al., 2008)

The wing phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 and the wing phenotype caused by expression of dpp^Scer\UAS.cHa under the control of Scer\GAL4^A9 is partially suppressed if the flies are also coexpressing tkv^{1ΔGSK.Scer\UAS}.

(Haerry et al., 1998)

Co-expression of one copy of tkv^{1ΔGSK.Scer\UAS} partially suppresses the wing phenotype caused by scw^Scer\UAS.cNa or dpp^Scer\UAS.cHa expressed under the control of Scer\GAL4^A9.

(Nguyen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tkv^{1ΔGSK.Scer\UAS}

tkv^1ΔGSK.UAS

Name Synonyms

Secondary FlyBase IDs

References (16)

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