FB2024_03 , released June 25, 2024
Allele: Scer\GAL4VP16.F442A.αTub84B
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General Information
Symbol
Scer\GAL4VP16.F442A.αTub84B
Species
S. cerevisiae
Name
FlyBase ID
FBal0090656
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GAL4-αTub84B(-FRT).VP16F442A}
Also Known As
Tubα1-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
FLPase
(Struhl and Greenwald, 2001, Wang et al., 1999)
in vitro construct
(Struhl and Adachi, 1998)
Progenitor genotype
Scer\GAL4VP16.F442A.FRT.αTub84B
(Jia et al., 2004, Struhl and Greenwald, 2001, Wang et al., 1999)
Carried in construct
P{GAL4-αTub84B(-FRT).VP16F442A}
Cytology
Description

αTub84B promoter drives expression of a GAL4::VP16 driver that includes the F442A substitution which reduces the activity of the Hsim\VP16 activation domain.

(Struhl and Adachi, 1998)
Allele components
Component
Use(s)
Encoded product / tool
GAL4::VP16
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism | ubiquitous
      (Struhl and Greenwald, 2001, Struhl and Adachi, 1998)
      larval stage
      organism | ubiquitous
      (Jia et al., 2004, Wang et al., 1999)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      αTub84B
      (Jia et al., 2004, Struhl and Greenwald, 2001, Wang et al., 1999, Struhl and Adachi, 1998)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The Scer\FRT cassette has been excised, juxtaposing the αTub84B promoter and Scer\GAL4-T:Hsim\VP16 fusion protein sequences.

      (Wang et al., 1999)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GAL4::VP16F442A.αTub84B
      Scer\GAL4F442A.αTub84B.T:Hsim\VP16
      Scer\GAL4VP16.F442A.αTub84B
      Name Synonyms
      Secondary FlyBase IDs
        References (8)