Expression of tinScer\UAS.cYa in the dorsal mesoderm in stage 11-12 embryos, driven by Scer\GAL4tin.cBa, does not induce the production of supernumerary cardioblasts.
Ectopic expression of tinScer\UAS.cYa in the lateral domain of the cardiogenic mesoderm, driven by either Scer\GAL4Mef2.PR or Scer\GAL4twi.PG, causes a marked reduction of lymph-gland progenitors and pericardial cells in stage 16 embryos.
When tinScer\UAS.cYa is expressed under the control of Scer\GAL4twi.PB the embryonic dorsal vessel is either distorted or does not form at all.
Stage 11 embryos expressing tinScer\UAS.cYa under the control of both Scer\GAL4how-24B and Scer\GAL4twi.PG have enlarged clusters of pericardial cell progenitors; each cluster contains 6-10 eve-expressing cells (e-PCs) compared to 3-4 cells per cluster in wild-type embryos. The number of e-PCs decreases to a normal level during subsequent stages and the heart appears to be normal.
tinUAS.cYa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Doc2UAS.cRa, Scer\GAL4tin.cBa
pnrUAS.cHa/tinUAS.cYa, Scer\GAL4twi.2PE is an enhancer of cardioblast | increased number phenotype of Doc2UAS.cRa, Scer\GAL4twi.2PE
pnrUAS.cHa/tinUAS.cYa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Doc2UAS.cRa, Scer\GAL4tin.cBa
tinUAS.cYa/Doc2UAS.cRa, Scer\GAL4tin.cBa is an enhancer of cardioblast | increased number phenotype of Scer\GAL4tin.cBa, pnrUAS.cHa
Scer\GAL4tin.cBa/tinUAS.cYa is an enhancer of cardioblast | increased number phenotype of Scer\GAL4tin.cBa, pnrUAS.cHa
Expression of tinScer\UAS.cYa, driven by Scer\GAL4tin.cBa, results in the rescue of few additional cardioblasts in a Df(3L)DocA background. Coexpression of pnrScer\UAS.cHa and tinScer\UAS.cYa, under the control of Scer\GAL4tin.cBa, induces the formation of more cardioblasts than expression of pnrScer\UAS.cHa alone. When Doc2Scer\UAS.cRa is coexpressed with only tinScer\UAS.cYa, or pnrScer\UAS.cHa and tinScer\UAS.cYa together, large numbers of extra cardioblasts are formed. Expression of all three transgenes results in double the amount of cardioblasts compared to wild type. Coexpression of Doc2Scer\UAS.cRa, tinScer\UAS.cYa, and pnrScer\UAS.cHa, under the control of Scer\GAL4twi.2PE, causes widespread ectopic cardioblast formation to a much greater extent than expression of Doc2Scer\UAS.cRa alone or in combination with tinScer\UAS.cYa plus pnrScer\UAS.cHa.