FB2024_03 , released June 25, 2024
Allele: Ecol\lacZMef2.175.wt
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General Information
Symbol
Ecol\lacZMef2.175.wt
Species
E. coli
Name
FlyBase ID
FBal0086486
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{Mef2-lacZ.175.wt}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Cripps et al., 1998)
Progenitor genotype
Carried in construct
P{Mef2-lacZ.175.wt}
Cytology
Description

A 175bp fragment consisting of Mef2 sequences from -2421bp to -2247bp is fused upstream of a heat shock promoter driving expression of a lacZ reporter.

(Cripps et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
Mef2_adepithelial_enhancer
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 9 -- 10
      somatic mesoderm
      (Cripps et al., 1998)
      embryonic stage 11 -- 15
      embryonic myoblast | segmentally repeated

      Comment: expression levels substantially decreased at stage 15

      (Cripps et al., 1998)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Mef2
      (Cripps et al., 1998)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid, transfected into SL2 cells with a twi expression vector and assayed for expression.

      (Cripps et al., 1998)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZMef2.175.wt
      lacZMef2.175.wt
      Name Synonyms
      Secondary FlyBase IDs
        References (4)