Homozygous clones on the metanotum can give rise to an ectopic structure that has pedicel-like sensilla campaniformia at its base (these sensory structures are normally found in the basal part of the haltere). Homozygous clones can also give rise to invaginations of the notum cuticle, and some of these invaginations have trichomes and hinge-like sensilla campaniformia.
Mutants show no overt defects in the overall architecture of the CNS.
tupunspecified mutant embryos have a broad head. Germ band shortening ceases early, resulting in the three most posterior segments remaining on the dorsal side of the embryo.
tupunspecified is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4Lim3.B, vvlUAS.cCa
exexunspecified, tupunspecified has abnormal neuroanatomy phenotype
tupunspecified is a suppressor of larval transverse nerve | embryonic stage 16 phenotype of Scer\GAL4Lim3.B, vvlUAS.cCa
exexunspecified, tupunspecified has larval longitudinal connective phenotype
exexunspecified, tupunspecified has larval intersegmental nerve phenotype
In tupunspecified, exexunspecified double mutants the scaffold of CNS axons is aberrant, the commissures are often greatly reduced or absent. ISNb projections clump in the proximity of the ISN. Axons within the ISN defasciculate aberrantly and ISNs from adjacent hemisegments can fuse across the parasegment boundary.