FlyBase Allele Report: Dmel\eya[UAS.cPa]

General Information

Symbol

Dmel\eya^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pignoni

FlyBase ID

FBal0085855

Feature type

allele

Associated gene

Dmel\eya

Associated Insertion(s)

Carried in Construct

P{UAS-eya.P}

Also Known As

UAS-eya

Key Links

Transgenic product class

cDNA

(Pignoni et al., 1997)

wild_type

(Pignoni et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pignoni et al., 1997)

wild_type

(Pignoni et al., 1997)

Mutagen

in vitro construct

(Chen et al., 1997, Pignoni et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-eya.P}

Cytology

Description

eya type II cDNA is expressed under the control of UASt regulatory sequences.

(Pignoni et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

eya

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^dpp.blk1

(Chen et al., 1997, Pignoni et al., 1997)

Phenotype Manifest In

adult head, with Scer\GAL4^dpp.blk1

(Chen et al., 1997)

antenna, with Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

antennal disc, with Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

eye | ectopic, with Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004, Chen et al., 1997)

leg, with Scer\GAL4^dpp.blk1

(Chen et al., 1997, Pignoni et al., 1997)

ommatidium | ectopic, with Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

wing, with Scer\GAL4^dpp.blk1

(Chen et al., 1997, Pignoni et al., 1997)

Detailed Description

Statement

Reference

Expression of eya^Scer\UAS.cPa under the control of Scer\GAL4^30A does not result in the induction of ectopic retinal cells in the wing disc.

(Chen et al., 1999)

Ectopic expression of eya^Scer\UAS.cPa using Scer\GAL4^dpp.blk1 produces very small ectopic eyes on the head ventral to the antennae in 34% of flies. A tiny spot of red pigment is seen at the joint between the coxa and femur of the leg in 95% of cases, and on the wing blade in 26% of cases.

(Chen et al., 1997)

Ectopic expression of eya^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 often causes mild growth alterations in the antennal disc, resulting in extra folds in the epithelium and, rarely, formation of small ectopic ommatidial arrays. Very small patches of red pigment cells are often induced on the antennae, wings and legs in these flies.

(Pignoni et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cPa has visible phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of visible phenotype of Scer\GAL4^dpp.blk1, dac^UAS.cSa

(Chen et al., 1997)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has visible phenotype

(Chen et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{ΔDD1.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{ΔDD2.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by Scer\GAL4^dpp.blk1/dac^{ΔN.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{ΔNLS2.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{N.DD1.DD2.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{N.DD1.M.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^UAS.Tag:HA/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{ΔM.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, enhanceable by dac^{ΔC.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has wing disc phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^30A

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has photoreceptor neuron | ectopic phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^30A

(Pappu et al., 2003)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has antennal disc phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has leg phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has antenna phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has wing phenotype, enhanceable by so^UAS.cPa/Scer\GAL4^dpp.blk1

(Pignoni et al., 1997)

NOT Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, non-enhanceable by dac^{ΔDD1.ΔDD2.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, non-enhanceable by dac^{N.DD1.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1, eya^UAS.cPa has eye | ectopic phenotype, non-enhanceable by dac^{N.DD2.UAS.Tag:HA}/Scer\GAL4^dpp.blk1

(Tavsanli et al., 2004)

Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔC.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔDD1.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔDD2.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔN.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔNLS2.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{N.DD1.DD2.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{N.DD1.M.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^UAS.Tag:HA

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔM.UAS.Tag:HA}

(Tavsanli et al., 2004)

eya^UAS.cPa/Scer\GAL4^30A is an enhancer of wing disc phenotype of Scer\GAL4^30A, ey^UAS.cHa

(Chen et al., 1999)

NOT Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/eya^UAS.cPa is a non-enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{ΔDD1.ΔDD2.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^dpp.blk1/eya^UAS.cPa is a non-enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, dac^{N.DD2.UAS.Tag:HA}

(Tavsanli et al., 2004)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, eya^UAS.cPa is a suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, eya^UAS.cPa is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Other

Statement

Reference

Scer\GAL4^30A, ey^UAS.cHa, eya^UAS.cPa, hh^UAS.cCa has wing disc phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, ey^UAS.cHa, eya^UAS.cPa, hh^UAS.cCa has photoreceptor neuron | ectopic phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has wing disc phenotype

(Pappu et al., 2003)

Scer\GAL4^30A, dpp^UAS.cSa, ey^UAS.cHa, eya^UAS.cPa has photoreceptor neuron | ectopic phenotype

(Pappu et al., 2003)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has wing phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has adult head phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has eye-antennal disc phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has wing disc phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has leg disc phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has dorsal mesothorax phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has femur | dorsal phenotype

(Chen et al., 1997)

Scer\GAL4^dpp.blk1, dac^UAS.cSa, eya^UAS.cPa has tibia | dorsal phenotype

(Chen et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

When both dac^{Scer\UAS.T:Ivir\HA1} and eya^Scer\UAS.cPa are driven by Scer\GAL4^dpp.blk1, they act synergistically on the ectopic eye phenotype to form large patches of ectopic eye tissue ventral to the antenna that often fuse and expands the wild-type eye. Ectopic eyes are also seen on the leg and thorax. When both dac^{ΔN.Scer\UAS.T:Ivir\HA1}, dac^{ΔN.Scer\UAS.T:Ivir\HA1}, dac^{ΔNLS2.Scer\UAS.T:Ivir\HA1}, dac^{ΔM.Scer\UAS.T:Ivir\HA1}, dac^{ΔC.Scer\UAS.T:Ivir\HA1}, dac^{N.DD1.DD2.Scer\UAS.T:Ivir\HA1}, or dac^{N.DD1.M.Scer\UAS.T:Ivir\HA1} and eya^Scer\UAS.cPa are driven by Scer\GAL4^dpp.blk1, they act synergistically on the ectopic eye phenotype. Larger and more penetrant ectopic eye formation is seen ventral to the antenna. When both dac^{ΔDD1.Scer\UAS.T:Ivir\HA1} and eya^Scer\UAS.cPa are driven by Scer\GAL4^dpp.blk1, they act synergistically on the ectopic eye phenotype. Red pigmentation is seen on the dorsolateral side of the antenna with no ommatidia is seen. When both dac^{N.DD2.Scer\UAS.T:Ivir\HA1}, dac^{ΔDD1.ΔDD2.Scer\UAS.T:Ivir\HA1}, or dac^{N.DD1.Scer\UAS.T:Ivir\HA1}and eya^Scer\UAS.cPa are driven by Scer\GAL4^dpp.blk1, no enhancement of the ectopic eye phenotype is seen.

(Tavsanli et al., 2004)

Clones of so^Scer\UAS.cPa; eya^Scer\UAS.cPa; Scer\GAL4^Act5C.PP cells in the testes do not induce precocious spermatocyte development.

(Fabrizio et al., 2003)

When dpp^Scer\UAS.cSa, eya^Scer\UAS.cPa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in both compartments of the wing disc. The addition of so^Scer\UAS.cPa increases the penetrance of this phenotype. When hh^Scer\UAS.cCa, eya^Scer\UAS.cPa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment. The addition of dpp^Scer\UAS.cSa or eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also not affected. The addition of so^Scer\UAS.cPa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc has no effect on the photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also not affected. The addition of dpp^Scer\UAS.cSa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued.

(Pappu et al., 2003)

Enhances the ectopic retinal development phenotype in the wing disc caused by expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^30A.

(Chen et al., 1999)

Coexpression of eya^Scer\UAS.cPa and dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 induces substantial ectopic eyes on the head, legs, wings and dorsal thorax. The cuticle between the normal eye field and the antenna is transformed into retinal cells such that the normal retinal field is expanded. Large patches of pigment are induced on the dorsal side of the femur and tibia of all legs, which are severely truncated. Ommatidial structures are observed in these patches. Red pigment, but no clear ommatidial morphology, is induced on the wing blade. Ectopic eyes are induced bilaterally on the dorsal thorax. All these phenotypes have 100% penetrance. Coexpression of eya^Scer\UAS.cPa and dac^Scer\UAS.cSa using Scer\GAL4^dpp.blk1 induces ectopic morphogenetic furrow advancement from the ventral side of the eye in late larval eye-antennal discs. Substantial ectopic photoreceptor development is also seen. The axons of these ectopic photoreceptors form a bundle that extends posteriorly into the eye imaginal disc. These axons appear to fuse with the axon tracts sent out by photoreceptors of the normal retinal field and probably exit through the optic stalk and synapse with the larval brain. Ectopic neurons are induced in leg and wing discs. Axons extended by these neurons retract during late larval and pupal development.

(Chen et al., 1997)

Coexpression of both eya^Scer\UAS.cPa and so^Scer\UAS.cPa under the control of Scer\GAL4^dpp.blk1 results in a dramatic increase in the development of eye tissue in antennal discs compared to expression of eya^Scer\UAS.cPa alone under the control of Scer\GAL4^dpp.blk1. This tissue develops into adult eye structures. There is also an increase in the frequency and size of red pigmented patches on the wings and legs. Ectopic eyes are not produced when eya^Scer\UAS.cPa and so^Scer\UAS.cPa are coexpressed under the control of Scer\GAL4^dpp.blk1 in an ey² background, although growth alterations and patches of red pigment cells on the legs are still seen.

(Pignoni et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

eya^UAS.cPa/Scer\GAL4^h-1J3 partially rescues eya^cli-IID

(Suzuki and Saigo, 2000)

Scer\GAL4^upd1-E132/eya^UAS.cPa partially rescues eya²

(Pignoni et al., 1997)

Scer\GAL4^dpp.blk1/eya^UAS.cPa partially rescues eya²

(Pignoni et al., 1997)

Comments

The lack of Bolwig's organ neurons seen in eya^cli-IID embryos is partially rescued by eya^Scer\UAS.cPa expressed under the control of Scer\GAL4^h-1J3.

(Suzuki and Saigo, 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

cli^Scer\UAS.cPa

cli^UAS.cPa

eya^Scer\UAS.cPa

eya^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pignoni

(Pignoni et al., 1997)

Secondary FlyBase IDs

References (13)

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