FlyBase Allele Report: Dmel\Btk[fic-P]

General Information

Symbol

Dmel\Btk^fic-P

Species

D. melanogaster

Name

fickle-P

FlyBase ID

FBal0060769

Feature type

allele

Associated gene

Dmel\Btk

Associated Insertion(s)

P{BmΔ-w}Btk^fic-P

Carried in Construct

Also Known As

Btk29A^ficP, fic^P

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

P-element activity

(Yamamoto et al., 1997)

Delta2-3 transposase

(Baba et al., 1999)

Progenitor genotype

Associated Insertion(s)

P{BmΔ-w}Btk^fic-P

Cytology

Description

Insertion at 2L:8277721 (release 5 genome).

(Nawaz et al., 2012)

Insertion of P{BmΔ-w} into a PstI-EcoRI fragment that falls within an intron of the Type 2 Btk29A splice variant. Expression of the Type 2 transcript isoform is prevented but expression of the Type 1 transcript isoform is unaffected.

(Baba et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,277,721..8,277,728

(Nawaz et al., 2012, Baba et al., 1999, Yamamoto, 1997, Yamamoto et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Asztalos et al., 2007, Asztalos et al., 1997)

abnormal cell shape

(Hamada-Kawaguchi et al., 2015)

male sterile

(Baba et al., 1999)

short lived

(Hamada et al., 2005, Baba et al., 1999, Yamamoto et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant ovaries are grossly aberrant as a result of the irregular shapes and sizes of germ cells and follicle cells. Ring canals are much smaller than wild type - their lumina are very narrow.

(Hamada-Kawaguchi et al., 2015)

Btk29A^fic-P mutant germaria contain significantly more germ cells than do wild-type germaria. Although supernumerary cells with spectrosomes are observed in the mutants, many of the excess cells appear to be cystocytes, as they are accompanied by a branched fusome structure.

(Hamada-Kawaguchi et al., 2014)

The initial jump score of mutant flies in an olfactory jump reflex assay is normal. However, habituation of the olfactory jump reflex occurs significantly faster than normal in mutant flies. Spontaneous recovery of the olfactory jump reflex after habituation training is normal in the mutant flies. Dishabituation (after receiving a dishabituating stimulus) in mutant flies is not different from the response seen in wild-type flies. Sensitisation of the olfactory jump reflex is defective in mutant flies.

(Asztalos et al., 2007)

The apodemes in homozygous males typically split into two pieces at their base, in contrast to wild-type males which have a single apodeme. In some mutant males the single apodemes emerge at the base and then split proximally. Adult longevity is reduced to 11% of that of wild-type flies.

(Hamada et al., 2005)

Copulation involving mutant Btk29A^fic-P males often terminates shortly after initiation. Mating behavior of Btk29A^fic-P females is normal. Multiple copulation attempts occur though are rare in the wild type. Two apodemes are present bilaterally at the base of the penis apparatus in all mutant males (Btk29A^fic-P homozygotes and Btk29A^fic-P/Btk29A^L1-3), whereas wild type males have a single apodeme. Mutant males exhibit prominent atrophy of the posterior ejaculatory duct. Life span of mutant adults is reduced compared to wild-type flies.

(Baba et al., 1999)

Analysis of the non-associative learning of the jump reflex demonstrates mutants exhibit specific defects in sensitization which leads to fast habituation.

(Asztalos et al., 1997)

Flies exhibit a highly variable copulatory span and repetitive copulation in mated pairs often occurs soon after separation. Adult lifespan is decreased relative to wild-type.

(Yamamoto et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Btk^fic-P has short lived phenotype, suppressible by Hsap\BTK^hs.PH

(Hamada et al., 2005)

Suppressor of

Statement

Reference

Btk^k05610/Btk^fic-P is a suppressor of grandchildless phenotype of pcs^gs

(Sinka et al., 2002)

Btk^fic-P/Btk^fic-P is a suppressor of grandchildless phenotype of pcs^gs

(Sinka et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Btk^fic-P has nurse cell ring canal phenotype, suppressible by pcs^gs

(Hamada-Kawaguchi et al., 2015)

Btk^fic-P has phallapodeme phenotype, suppressible by Hsap\BTK^E41K.hs

(Hamada et al., 2005)

Btk^fic-P has phallapodeme phenotype, suppressible by Hsap\BTK^hs.PH

(Hamada et al., 2005)

Btk^fic-P has phallapodeme phenotype, suppressible by pcs^gs

(Hamada et al., 2005)

NOT suppressed by

Statement

Reference

Btk^fic-P has phallapodeme phenotype, non-suppressible by Hsap\BTK^mut.hs

(Hamada et al., 2005)

Suppressor of

Statement

Reference

Btk^fic-P is a suppressor of nurse cell ring canal phenotype of pcs^gs

(Hamada-Kawaguchi et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

A significant proportion of Btk29A^fic-P pcs^gs double mutant males develop normal looking genitalia.

(Hamada et al., 2005)

Xenogenetic Interactions

Statement

Reference

The split apodeme phenotype seen in Btk29A^fic-P males is partially rescued by expression of Hsap\BTK^hs.PH or Hsap\BTK^E41K.hs during the third larval instar and pupal stages, but is not rescued by expression of Hsap\BTK^mut.hs. The reduced longevity seen in Btk29A^fic-P adult flies is slightly, but significantly, alleviated by expression of Hsap\BTK^hs.PH during the third larval instar to pupal stage.

(Hamada et al., 2005)

Complementation and Rescue Data

Rescued by

Btk^fic-P is rescued by Btk^UAS.cHa/Scer\GAL4^bab1-GAL4-U

(Hamada-Kawaguchi et al., 2014)

Btk^fic-P is rescued by Btk^2.hs

(Baba et al., 1999)

Btk^fic-P is rescued by Btk^1.hs

(Baba et al., 1999)

Partially rescued by

Btk^fic-P is partially rescued by Btk^2.hs

(Hamada et al., 2005)

Btk^fic-P is partially rescued by Btk^1.hs

(Hamada et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Kyoto

109037

w¹¹¹⁸; P{BmΔ-w}Btk^fic-P / CyO

109098

w^* ; P{BmΔ-w}Btk^fic-P / CyO ; P{hs-hBtk^E41K}DY1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Btk29A<up> ficP</up>

(Hamada-Kawaguchi et al., 2014)

Btk29A^fic-P

Btk29A^ficP

(Hamada-Kawaguchi et al., 2015, Hamada-Kawaguchi et al., 2014, Nawaz et al., 2012, Hamada et al., 2005)

Btk29A^ficPL

(Sinka et al., 2002)

Btk^fic-P

fic^P

(Lu et al., 2004, Baba et al., 1999, Yamamoto, 1997, Yamamoto et al., 1997)

fickle^P

(Asztalos et al., 2007, Baba et al., 1997)

Name Synonyms

fickle

(Asztalos et al., 2007, Asztalos et al., 1997)

fickle-P

(Yamamoto et al., 1997)

Secondary FlyBase IDs

References (13)

Report Sections

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