FlyBase Allele Report: Dmel\Raf[DN.UAS]

General Information

Symbol

Dmel\Raf^DN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0059973

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{UAS-raf^DN}

Also Known As

UAS-Raf^DN

Key Links

Transgenic product class

dominant_negative_variant

(de Celis, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(de Celis, 1997)

Mutagen

in vitro construct

(de Celis, 1997)

Progenitor genotype

Carried in construct

P{UAS-raf^DN}

Cytology

Description

UAS regulatory sequences drive expression of a dominant negative form of Raf.

(de Celis, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Raf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by Ras85D^V12.UAS

(Zoranovic et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Phenotype Manifest In

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^bs-23.26

(Gallio et al., 2004)

wing, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

wing vein, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Detailed Description

Statement

Reference

Expressing Raf^DN.UAS under the control of Scer\GAL4^Bx-MS1096 results in a reduced wing size and loss of vein material.

(Kushnir et al., 2020)

Migration of many of the ganglionic tracheal branches is stalled or misrouted in phl^DN.Scer\UAS; Scer\GAL4^bs-23.26 embryos, but branches do not cross the ventral midline.

(Gallio et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible | partially by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, suppressible by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has abnormal size | adult stage phenotype, suppressible by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Other

Statement

Reference

Raf^DN.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has visible | adult stage phenotype

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096, rl^{D334N.UAS.cKa} has visible | adult stage phenotype

(Kushnir et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing vein phenotype, suppressible | partially by rl^R80S.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing vein phenotype, suppressible | partially by rl^WT.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing vein phenotype, suppressible by rl^{D334N.UAS.cKa}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096 has wing vein phenotype, suppressible by rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Suppressor of

Statement

Reference

Raf^DN.UAS, Scer\GAL4^C-580 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^C-580, tkv^{Q253D.UAS.cLa}

(de Celis, 1997)

Raf^DN.UAS, Scer\GAL4^bs-1348 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^bs-1348, tkv^{Q253D.UAS.cLa}

(de Celis, 1997)

Other

Statement

Reference

Raf^DN.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has wing vein phenotype

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096, rl^{D334N.UAS.cKa} has wing vein phenotype

(Kushnir et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

The wing defects induced by the expression of Raf^DN.UAS under the control of Scer\GAL4^Bx-MS1096 (smaller wing and loss of L3-5 vein material are: fully suppressed by the co-expression of either rl^{D334N.UAS.cKa} or rl^{R80S.D334N.UAS}, even leading to extra vein material (more severe in the latter, with darker pigmentation); and partially suppressed by the co-expression of rl^R80S.UAS or rl^WT.UAS (partial rescue of the proximal part of L3 and distal part of L4, or complete rescue of L5).

(Kushnir et al., 2020)

Reduces but does not completely suppress the ectopic wing vein formation seen in tkv^{Q253D.Scer\UAS.cLa} Scer\GAL4^bs-1348 flies, when expressed using Scer\GAL4^bs-1348.

(de Celis, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Raf^DN.Scer\UAS

Raf^DN.UAS

phl^DN.Scer\UAS

phl^DN.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close