FlyBase Allele Report: Dmel\kuz[UAS.cFa]

General Information

Symbol

Dmel\kuz^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Fambrough

FlyBase ID

FBal0057208

Feature type

allele

Associated gene

Dmel\kuz

Associated Insertion(s)

Carried in Construct

P{UAS-kuz.F}

Also Known As

UAS-kuz, UAS-KUZ-F

Key Links

Transgenic product class

cDNA

(Fambrough et al., 1996)

wild_type

(Fambrough et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Fambrough et al., 1996)

wild_type

(Fambrough et al., 1996)

Mutagen

in vitro construct

(Sotillos et al., 1997, Fambrough et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-kuz.F}

Cytology

Description

A 4.9kb kuz cDNA fragment, containing the entire coding region, is expressed under the control of UASt regulatory sequences.

(Fambrough et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kuz

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Appl^{UAS.mRFP.EGFP}

(Long et al., 2024)

exacerbates Alzheimer's disease

modeled by Appl^d

(Bourdet et al., 2015)

ameliorates cancer

modeled by Pvr^{λ.UASp.Tag:MYC}

(Wang et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Wild-type flies expressing kuz^Scer\UAS.cFa under the control of Scer\GAL4^{Mef2.247.Switch} exhibit wild-type memory performance, indicating that expression of kuz^Scer\UAS.cFa in the mushroom bodies has no impact on short-term memory.

(Bourdet et al., 2015)

Expression of kuz^Scer\UAS.cFa in egg chambers (under the control of Scer\GAL4^slbo.2.6) has no detectable effect.

(Wang et al., 2007)

Expression of kuz^Scer\UAS.cFa under the control of Scer\GAL4^slbo.2.6 does not result in defects in border follicle cell migration.

(Wang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

kuz^UAS.cFa/Scer\GAL4^{Mef2.247.Switch} is an enhancer of abnormal memory phenotype of Appl^d

(Bourdet et al., 2015)

NOT Enhancer of

Statement

Reference

kuz^UAS.cFa/Scer\GAL4^{Mef2.247.Switch} is a non-enhancer of abnormal learning phenotype of Appl^d

(Bourdet et al., 2015)

Suppressor of

Statement

Reference

kuz^UAS.cFa, Scer\GAL4^lz-gal4 is a suppressor of abnormal cell shape | larval stage phenotype of Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^lz-gal4

(Wang et al., 2023)

kuz^UAS.cFa/Scer\GAL4^Appl.G1a is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

NOT Suppressor of

Statement

Reference

kuz^UAS.cFa/Scer\GAL4^elav.PLu is a non-suppressor of abnormal neuroanatomy phenotype of tok^K788/tok^D427

(Meyer and Aberle, 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

kuz^UAS.cFa, Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell | larval stage phenotype of Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^lz-gal4

(Wang et al., 2023)

kuz^UAS.cFa/Scer\GAL4^Appl.G1a is a suppressor of embryonic/larval brain | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

NOT Suppressor of

Statement

Reference

kuz^UAS.cFa/Scer\GAL4^elav.PLu is a non-suppressor of embryonic/larval neuromuscular junction phenotype of tok^K788/tok^D427

(Meyer and Aberle, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of kuz^Scer\UAS.cFa in a Appl^d/+; Scer\GAL4^{Mef2.247.Switch} heterozygous background exacerbates the short term memory deficit found in this background. These flies exhibit this exacerbated defect regardless of whether the flies are fed with RU for 48 hours before conditioning. Appl^d/+; Scer\GAL4^{Mef2.247.Switch}/kuz^Scer\UAS.cFa flies fed with RU and testing immediately after conditioning exhibit wild-type learning. The ability to avoid electric shocks as well as their olfactory acuity to each odour after electric shock exposure is unaffected.

(Bourdet et al., 2015)

Expression of kuz^Scer\UAS.cFa under the control of Scer\GAL4^Appl.G1a suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

(Wentzell et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/kuz^UAS.cFa rescues kuz^unspecified

(Fambrough et al., 1996)

Partially rescues

kuz^UAS.cFa/Scer\GAL4^C-765 partially rescues kuz^k01405

(Sotillos et al., 1997)

Fails to rescue