FB2024_03 , released June 25, 2024
Allele: Ecol\lacZαTub84B.PHa
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General Information
Symbol
Ecol\lacZαTub84B.PHa
Species
E. coli
Name
α-Tubulin at 84B promoter construct a of Harrison
FlyBase ID
FBal0057052
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
P{CXY21.29+33}X15
Carried in Construct
P{CXY21.29+33}
Also Known As
tub-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
FLPase
(Ohlstein and Spradling, 2007, Ohlstein and Spradling, 2006, Drummond-Barbosa and Spradling, 2004, Song and Xie, 2002, Tworoger et al., 1999, Margolis and Spradling, 1995, Harrison and Perrimon, 1993)
Progenitor genotype
P{5'CXY21.29}X15
(Ohlstein and Spradling, 2006, Drummond-Barbosa and Spradling, 2004, Zhu and Xie, 2003)
Ecol\lacZX15-29
(Song and Xie, 2002, Drummond-Barbosa and Spradling, 2001, Margolis and Spradling, 1995, Harrison and Perrimon, 1993)
P{3'CXY21.33}X15
(Ohlstein and Spradling, 2006, Drummond-Barbosa and Spradling, 2004, Zhu and Xie, 2003, Song and Xie, 2002, Drummond-Barbosa and Spradling, 2001)
Carried in construct
P{CXY21.29+33}
Associated Insertion(s)
P{CXY21.29+33}X15
Cytology
Description

αTub84B promoter sequences drive expression of lacZ that is tagged at the N-terminal end with Tag:NLS(SV40-largeT). Reconstituted in vivo by FLP-mediated recombination between the FRT sites present in the deletion derivatives P{5'CXY21.29}X15 and P{3'CXY21.33}X15.

(Harrison and Perrimon, 1993)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
lacZ
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      organism | ubiquitous
      (Zhang and Kalderon, 2000)
      Additional Information
      Statement
      Reference

      Used as marker for mosaic analysis in embryos.

      (Zhang and Kalderon, 2000)
       
      Marker for
      Reflects expression of
      αTub84B
      (Zhang and Kalderon, 2000)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Exists in clones, not reported as being germ-line transmitted.

      (Margolis and Spradling, 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZαTub84B.PHa
      lacZαTub84B.PHa
      Name Synonyms
      α-Tubulin at 84B promoter construct a of Harrison
      (Harrison and Perrimon, 1993)
      Secondary FlyBase IDs
        References (13)